Since November of 2015, when ore tailings from a dam rupture reached the Atlantic Ocean, researchers are trying to assess the degree of impact across the Doce River and adjacent coastal area. This study aims to use the zooplankton dynamics as a tool to evaluate the environmental impact in the coastal region, five years after the rupture, during periods of low and high river flow. Doce River flow varied from 49 to 5179 m ³/s and structured the zooplankton community between periods of low and high river flow, but salinity and chlorophyll-a had stronger correlation with depth (r = 0.40 and − 0.40 respectively) than with the Doce River discharge variation along the sampling period (r < 0.2). On the other hand, inorganic particles in the water and total metal concentration (dissolved + particulate), used as tracers of the iron enriched tailing (Al, Cd, Cr, Cu, Fe, V), were correlated with fluvial discharge and showed to be the main factor driving the zooplankton community dynamics. For assessing the degree of environmental impact, we tested the ecological indexes for the zooplankton community. Margalef Richness, Pielou Evenness and Shannon-Wiener Diversity varied from 2.52, 0.40 and 1.39 (all registered during high river flow period) to 9.02, 0.85 and 3.44 (all registered during low river flow period), respectively. Along with those community indicators, we evaluated the response of representative taxonomical genera such as Paracalanus, Oikopleura and Temora, regarding the Doce River flow, and found population patterns that established a baseline for future monitoring in the region. Our results showed that the zooplankton community is more fragile when the river discharge is stronger, and this pattern is confirmed by all indicators tested.

Zooplankton were sampled five days after the tailings from the Samarco dam rupture reached the ocean in the coastal region at the mouth of the Doce River. This was one of the largest environmental disasters in Brazilian history, and the impacts on the marine biota are not yet fully understood. This study aimed to evaluate the zooplankton community short term responses to the metal enrichment after the tailings reached the coastal region in different scenarios. Our results showed an acute impact on the zooplankton community, which peaked in abundance (222,958.60 ind/m ³) and decreased in diversity (H′ = 1.23) near the river mouth. Two copepod species, Parvocalanus sp. and Oithona nana, composed up to 61% of the total abundance and they were correlated with concentrations of Fe, Pb, Cu and Zn in particulate fraction. These species feed opportunistically on nanophytoplankton, which dominated the autotroph community, possibly in response to the iron enrichment caused by the mud flow. A shift on zooplankton species composition was also observed. During the first three days, we found the presence of oceanic species in the 20 and 30 m isobaths during an incomplete upwelling event, which directly correlated with the presence of Calanoides carinatus. However, only three days later, following a cold front passage and consequent increase of water turbidity, those species were already absent, and the zooplankton community was significantly altered (PERMANOVA, df = 1, pseudo-F = 9.2247, p = .001). Zooplankton responded quickly to the environmental changes detected during our sampling period and proved to be key factors in costal monitoring, especially in dynamic oceanographic areas such as the Doce River coastal region.