CB
Carlijn V. C. Bouten
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2 records found
1
Journal article
(2023)
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Janine Grolleman, Nicole C. A. van Engeland, Minahil Raza, Sepinoud Azimi , Vito Conte, Cecilia M. Sahlgren, Carlijn V. C. Bouten
Recent experimental evidence indicates a role for the intermediate filament vimentin in regulating cellular mechanical homeostasis, but its precise contribution remains to be discovered. Mechanical homeostasis requires a balanced bi-directional interplay between the cell’s microenvironment and the cellular morphological and mechanical state—this balance being regulated via processes of mechanotransduction and mechanoresponse, commonly referred to as mechanoreciprocity. Here, we systematically analyze vimentin-expressing and vimentin-depleted cells in a swatch of in vitro cellular microenvironments varying in stiffness and/or ECM density. We find that vimentin-expressing cells maintain mechanical homeostasis by adapting cellular morphology and mechanics to micromechanical changes in the microenvironment. However, vimentin-depleted cells lose this mechanoresponse ability on short timescales, only to reacquire it on longer time scales. Indeed, we find that the morphology and mechanics of vimentin-depleted cell in stiffened microenvironmental conditions can get restored to the homeostatic levels of vimentin-expressing cells. Additionally, we observed vimentin-depleted cells increasing collagen matrix synthesis and its crosslinking, a phenomenon which is known to increase matrix stiffness, and which we now hypothesize to be a cellular compensation mechanism for the loss of vimentin. Taken together, our findings provide further insight in the regulating role of intermediate filament vimentin in mediating mechanoreciprocity and mechanical homeostasis.
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Recent experimental evidence indicates a role for the intermediate filament vimentin in regulating cellular mechanical homeostasis, but its precise contribution remains to be discovered. Mechanical homeostasis requires a balanced bi-directional interplay between the cell’s microenvironment and the cellular morphological and mechanical state—this balance being regulated via processes of mechanotransduction and mechanoresponse, commonly referred to as mechanoreciprocity. Here, we systematically analyze vimentin-expressing and vimentin-depleted cells in a swatch of in vitro cellular microenvironments varying in stiffness and/or ECM density. We find that vimentin-expressing cells maintain mechanical homeostasis by adapting cellular morphology and mechanics to micromechanical changes in the microenvironment. However, vimentin-depleted cells lose this mechanoresponse ability on short timescales, only to reacquire it on longer time scales. Indeed, we find that the morphology and mechanics of vimentin-depleted cell in stiffened microenvironmental conditions can get restored to the homeostatic levels of vimentin-expressing cells. Additionally, we observed vimentin-depleted cells increasing collagen matrix synthesis and its crosslinking, a phenomenon which is known to increase matrix stiffness, and which we now hypothesize to be a cellular compensation mechanism for the loss of vimentin. Taken together, our findings provide further insight in the regulating role of intermediate filament vimentin in mediating mechanoreciprocity and mechanical homeostasis.
Journal article
(2016)
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A. J.M. Giesbers, P. C.P. Bouten, J. F.M. Cillessen, L. Van Der Tempel, J. H. Klootwijk, A. Pesquera, A. Centeno, A. Zurutuza, A. R. Balkenende
In this work we present the effect of defects in graphene on its potential for application in flexible electronics. We visualize defects at the grain boundaries, transfer defects and local atomic defects. We show that these defects are currently determining the gas barrier properties of graphene. Under strain up to only 2% we show that these defects lead to cracks in the graphene thereby deteriorating its conductivity.
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In this work we present the effect of defects in graphene on its potential for application in flexible electronics. We visualize defects at the grain boundaries, transfer defects and local atomic defects. We show that these defects are currently determining the gas barrier properties of graphene. Under strain up to only 2% we show that these defects lead to cracks in the graphene thereby deteriorating its conductivity.