WW
Wolfgang Wiechert
3 records found
1
Authored
13C labeling experiments at metabolic nonstationary conditions
An exploratory study
Background: Stimulus Response Experiments to unravel the regulatory properties of metabolic networks are becoming more and more popular. However, their ability to determine enzyme kinetic parameters has proven to be limited with the presently available data. In metabolic flux ana
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Two new concepts, "Limitation Potential" and "Constraint Limitation Sensitivity" are introduced that use definitions derived from metabolic flux analysis (MFA) and metabolic network analysis (MNA). They are applied to interpret a measured flux distribution in the context of al ...
New Tools for Mass Isotopomer Data Evaluation in 13C Flux Analysis
Mass Isotope Correction, Data Consistency Checking, and Precursor Relationships
13C metabolic flux analysis (MFA) is based on carbon-labeling experiments where a specifically 13C labeled substrate is fed, The labeled carbon atoms distribute over the metabolic network and the label enrichment of certain metabolic pools is measured by ...