This study examines how sustainable commuting alternatives can be designed and implemented to maximise adoption among hospital employees, particularly considering non-standard employment schedules and location-specific challenges. Conducted as part of the 'Living Lab Sustainable Transport' initiative, the research aims to provide insights for the Academic Medical Center and Pon Mobility Nederland on how to maximise the adoption of eco-friendly commuting options, while addressing the unique challenges of shift work and irregular schedules. By analysing employee preferences for alternative commuting options, such as cycling, e-bike leasing, shared e-bikes and public transport, the study aims to offer practical recommendations for promoting a modal shift away from car-based commutes. A Discrete Choice Experiment (DCE) was conducted to assess key factors influencing commuting decisions, including financial incentives, travel time, and perceived comfort. Findings indicate that employees with irregular schedules require additional support, particularly regarding safety concerns and flexibility for off-peak commutes. Financial incentives, such as full public transport reimbursement, e-bike travel allowances, and increased parking costs, were effective in encouraging more sustainable commuting behaviours. The results contribute valuable insights for the healthcare sector and other industries facing similar commuting challenges, offering a framework for promoting sustainable commuting practices while addressing the unique constraints of shift work and location-specific factors.