Urban freight logistics currently have to deal with multiple unsustainable conditions. Physical Internet (PI) characteristics show promise in making urban freight logistics more sustainable. This paper explores the opportunities and barriers to implementing this concept. Q-methodology is a method used to reveal different stakeholder perspectives. The results of the Q-methodology show four different perspectives out of which three display a positive attitude toward PI characteristics. One perspective is more moderate and states that a lot is possible already without any changes. One of the barriers is that there is no urgency to change. Further, most perspectives have a positive attitude toward regulations as long as they are nationally coordinated. Based on these results, policy recommendations are developed for individual and collaborative actions for stakeholders.