This study presents an AlN-based Lamb wave (A0 mode) liquid sensing device designed for biomedical applications. The Lamb wave device features a 1.5 μm composite membrane consisting of a 500 nm LPCVD SiN and a 1 μm of a c-axis oriented AlN film. Additionally, an innovative 45° rotated IDT design was explored to minimize edge reflections towards the output IDT. Liquid testing experiments, involving IPA, DI water, and D-PBS, were conducted to see if the devices were able to differentiate between these liquids. Results demonstrate that the fabricated Lamb wave devices exhibit sensitivity to mass loading and can distinguish between liquids based on phase, frequency, and gain characteristics. Devices with the rotated IDT design have shown a significant increase in resonance by 15 dB, as well as enhanced sensitivity compared to those with the normal design. Furthermore, devices with the rotated design demonstrated a higher Q factor of 680, indicating superior performance over the normal design (Q factor of 450). These findings suggest that a Lamb wave device with the 45° rotated IDT design holds considerable potential for applications that demand accurate liquid characterization and detection.