Bacillus nitratireducens was isolated from textile effluent and showed high tolerance to chromium (Cr), reaching up to a 1000 mg/L MIC value. This research was aimed at utilizing biosorbents from live and dead cells of B. nitratireducens to remove Cr from an aqueous solution. A batch biosorption test was performed, and mechanisms analysis was approached by an adsorption-desorption test, SEM-EDS, and FTIR analysis. Cr removal by dead cells in 25, 50, and 100 mg/L of Cr were 58.99 ± 0.7%, 69.8 ± 0.2%, and 82.87 ± 0.11%, respectively, while that by live cells was 73.08 ± 1.9%, 80.27 ± 6.33%, and 86.17 ± 1.93%, respectively. Live cells showed significantly higher Cr removal and adsorption capacities as compared to dead cells. In all concentrations, absorption contributed more than adsorption to the Cr removal by both live and dead cells. Absorption of Cr was subjected to occur due to passive mechanisms in dead cells while involving some active mechanisms in live cells. SEM-EDS confirmed the detection of Cr on the cell surface, while FTIR revealed the shifting of some peaks after the biosorption test, suggesting interactions between Cr and functional groups. Further TEM analysis is suggested to be conducted as a future approach to reveal the inner structure of cells and confirm the involvement of absorption mechanisms.