Governments are looking more and more to invest in renewable energy sources due to the energy transition that is currently taking place. One of the many renewable energy sources is wind energy which is increasingly positioned at sea. Wind turbines in deep parts of the ocean can be placed on floating structures which are often moored to the sea bottom by mooring ropes. For deep sea, the only viable option is a synthetic mooring line due to its almost neutral buoyancy. Mooring ropes have a vital role in the offshore floating structure as it is keeping the structure in place. When a mooring line breaks, the consequences may be big, leading to serious damage or dangerous situations. Therefore, the structural integrity of mooring ropes should be evaluated regularly. For synthetic mooring ropes, the only method at this point in time is visual inspection. This can be done by divers or by Remotely Operated Vehicles (ROVs). This method is expensive, time consuming and in case it is done by divers, it is potentially dangerous. Furthermore, synthetic mooring ropes are susceptible to external damage which means inspection would have to be executed without direct contact with the mooring ropes. Therefore, it is necessary to assess the feasibility of a non-contact, non-destructive testing method in order to assess the structural integrity of a synthetic mooring line. The combination of non-destructive material property assessment and tension assessment is believed to produce a structural health monitoring instrument for synthetic mooring ropes. In this thesis, a methodology is proposed which uses two independent non-contact, non-destructive measurements to assess the structural integrity of a high modulus polyethylene (HMPE) rope specimen. The measurements involved are ultrasonic guided wave (UGW) measurements and vibration measurements. The UGW measurements are performed to assess the stiffness of the test specimen according to the principle of attenuation of ultrasonic waves propagating through a specimen. The vibration measurements are performed to assess the natural frequencies of a manually excited test specimen. The assessed natural frequencies and the determined stiffness of the test specimen can be used to calculate the load acting on the test specimen. The methodology is tested by conducting experiments in a laboratory environment where in-situ conditions are recreated by performing the tests underwater. It was concluded that the loads can be recalculated with varying accuracy of approximately 10% with respect to the actual values, with increasing accuracy for higher load values. It is concluded that the proposed methodology has the potential to determine load on a synthetic mooring line in a non-contact, non-destructive manner.