It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca2+ signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca2+, ER Ca2+ release is the source of intracellular Ca2+ oscillations induced by laser-tweezer-traction at the plasma membrane, providing a model to study how mechanical stimuli can be transmitted deep inside the cell body. This ER Ca2+ release upon mechanical stimulation is mediated not only by the mechanical support of cytoskeleton and actomyosin contractility, but also by mechanosensitive Ca2+ permeable channels on the plasma membrane, specifically TRPM7. However, Ca2+ influx at the plasma membrane via mechanosensitive Ca2+ permeable channels is only mediated by the passive cytoskeletal structure but not active actomyosin contractility. Thus, active actomyosin contractility is essential for the response of ER to the external mechanical stimuli, distinct from the mechanical regulation at the plasma membrane.@en

In order to study the maintenance strategy of Epoxy Asphalt Concrete (EAC) in low temperature environment, the similarity of four anti-cracking performance indexes (plane strain fracture toughness, bending stiffness modulus, ultimate tensile stress and ultimate elongation linear strain) of EAC were analyzed, and the mathematical model for investigating the relationship between the number of freeze-thaw cycles and the comprehensive evaluation index of the crack resistance of epoxy asphalt mixture (EAM) was established in this paper. At first, the weight of each index is determined by one-way ANOVA. Then the anti-cracking performance of EAC under freezing-thawing condition is evaluated by fuzzy comprehensive evaluation method. The results show that the pavement maintenance bureau needs to carry out the maintenance of epoxy asphalt pavement on steel bridge deck every 30 days (1 month) for ensuring the crack resistance of EAC in winter with low temperature.@en

In order to evaluate the influence of different admixtures on the performance of cement stabilized macadam, the influence of fly ash, expansive agent, brucite fiber and alkali salt early strength agent on dry shrinkage, temperature shrinkage, compression and tensile properties of cement stabilized macadam mixture was studied by unconfined compressive strength test, dry shrinkage test, temperature shrinkage test and splitting test, respectively. The results show that the temperature shrinkage, dry shrinkage, unconfined compressive strength and Splitting strength of cement stabilized macadam with fly ash, expansive agent, brucite fiber and basic salt are greatly improved.@en

In order to evaluate the influence of different admixtures on the performance of cement stabilized macadam, the influence of fly ash, expansive agent, brucite fiber and alkali salt early strength agent on dry shrinkage, temperature shrinkage, compression and tensile properties of cement stabilized macadam mixture was studied by unconfined compressive strength test, dry shrinkage test, temperature shrinkage test and splitting test, respectively. The results show that the temperature shrinkage, dry shrinkage, unconfined compressive strength and Splitting strength of cement stabilized macadam with fly ash, expansive agent, brucite fiber and basic salt are greatly improved.@en