The native European flat oyster (Ostrea edulis) is an ecosystem engineer providing important ecosystem services, but became nearly extinct from the North Sea due to diseases and overfishing. There's a growing interest to restore these oyster reefs for their valuable contribution in re-establishing a rich ecosystem in the North Sea. In order to reintroduce the flat oyster population, the availability of hard substrate is crucial for initial settlement and reef development. Such substrate is offered by the infrastructure in offshore wind farms, by means of quarried rock placed at the base of the wind turbine foundations and on top of cable crossings to prevent scouring of the seabed. Further anthropogenic disturbances of the seabed are largely restricted, making wind farm areas promising sites for oyster reef restoration. For successful oyster reef initiation, offering a suitable type of substrate for larvae settlement is important. Here, we assess the settlement preference of flat oysters on 9 different types of substrate, by comparing total settlement, spat densities and spat survival. Oyster larvae settlement preference based on the total number of spat per surface area of the substrate was the highest for granite, a rock type conventionally used as scour protection in offshore wind farms. The lowest settlement preference was observed for steel and the biodegradable polymer BESE. The experiments were performed in a spatting pond and in a natural bay to be able to compare spat collection under controlled and natural conditions. Settlement rates in the spatting pond were much higher than in the natural environment, though survival rates were lower. Our results provide insight in the settlement preference of the European flat oyster for different types of substrate under controlled and natural conditions. Knowing these favorable substrates and conditions for oyster larvae settlement allows for the selection of pro-active measures that contribute to flat oyster reef restoration in the North Sea.

Diffractive shearing interferometry (DSI) is a method that has recently been developed to perform lensless imaging using extreme ultraviolet radiation generated by high-harmonic generation. In this paper, we investigate the uniqueness of the DSI solution and the requirements for the support constraint size. We find that there can be multiple solutions to the DSI problem that consist of displaced copies of the actual object. These alternative solutions can be eliminated by enforcing a sufficiently tight support constraint, or by introducing additional synthetic constraints. We furthermore propose a new DSI algorithm inspired by the analogy with coherent diffractive imaging (CDI) algorithms: the original DSI algorithm is in a way analogous to the hybrid input-output algorithm as used in CDI, and we propose a new algorithm that is more analogous to the error reduction algorithm as used in CDI. We find that the newly proposed algorithm is suitable for final refinement of the reconstruction.

We report on a method that allows microscopic image reconstruction from extreme-ultraviolet diffraction patterns without the need for object support constraints or other prior knowledge about the object structure. This is achieved by introducing additional diversity through rotation of an object in a rotationally asymmetric probe beam, produced by the spatial interference between two phase-coherent high-harmonic beams. With this rotational diffractive shearing interferometry method, we demonstrate robust image reconstruction of microscopic objects at wavelengths around 30 nm, using images recorded at only three to five different object rotations.