Runoff processes in glacier and páramo catchments in the Andean region are of interest as they are vitally important to serve the water needs of surrounding communities. Particularly in Northern Ecuador, the runoff processes are less well-known due to the high variability of precipitation, young volcanic ash soil properties, soil moisture dynamics and other local factors. Previous studies have shown that the melting of glaciers contributes to runoff generation and that the páramo ecosystem plays an important role in regulating runoff during periods of low precipitation. Data collection and experimental investigations were carried out in a catchment of 15.2 km² and altitude ranging between 4000 and 5700 m above sea level. Environmental tracers and hydrochemical catchment characterization were used for identifying runoff sources and their respective contributions during dry and wet conditions. Dry conditions are defined as periods where precipitation was absent for at least three consecutive days and wet conditions imply rainfall events. This study highlights the importance of the páramo on contributing to total runoff during baseflow (70% of total runoff) and the capacity of the páramo to dissipate the stream energy and buffer the peak flow during rainfall conditions. Electrical conductivity together with stable isotopes were identified as conservative tracers that characterize the end-member concentrations.