Highlights: What are the main findings? Multitemporal surface reflectance shows trends in oxbow lake area and water types. Oxbow lake connectivity peaks during the flooding period under natural conditions. What are the implications of the main findings? Connectivity-based groups of oxbow lakes represent the availability of fish habitat. Oxbow lake diversity and connectivity are essential for river ecological integrity. In meandering river floodplain systems, remote sensing is a valuable tool for assessing connectivity processes relevant to fish ecological functions. This study used the Google Earth Engine platform and multispectral Landsat 7 imagery. A random forest classifier was used to evaluate water types and area changes in oxbow lakes of the Beni River in Bolivia. Water type dynamics were mainly associated with lake age and distance from the main channel. Seasonal variations highlighted the role of wind-driven sediment resuspension and overflow during high discharge conditions. Long-term lake area changes reflected typical oxbow lake evolution as well as alterations caused by the main channel. Multiannual changes showed a notable area decrease during years of low discharge. Relationships between discharge and lake area dynamics allowed the classification of three lake groups with different levels of connectivity and overbank flow influence. The ecological relevance of these groups was evaluated based on fish habitat preferences and migration patterns. Results emphasize the importance of preserving natural hydrologic variability, with flooding associated with increased habitat availability. Overall, this study demonstrates the usefulness of satellite remote sensing for detecting ecohydrological processes and offers insights to preserve ecological functions in data-scarce regions.

The natural flow regimes of Andean-Amazon tributaries play a vital role in sustaining their rich biodiversity and productive local fisheries, but ongoing and proposed alteration of river flow regimes by large dams threatens to negatively impact river ecosystems. Despite its importance, our understanding of how hydrologic variability influences ecological functions in the Andean Amazon is limited, particularly in regions with scarce data. In these regions, growing research highlights the value of fishers' local ecological knowledge in addressing these gaps. This study focused on increasing our knowledge of ecohydrological relationships in the Beni River of Bolivia through the analysis of fishers' knowledge through 28 individual semi-structured interviews. Results indicate how key species rely on hydrologic variability, connectivity, and flooding dynamics to carry out their life stages of reproductive migration and access different habitats in the floodplains. Fishers mentioned using hydrologic indicators at multiple scales to guide their fishing activity. For instance, flooding extent and duration help anticipate fish abundance in the next years; connectivity between the main channel and oxbow lakes indicates fish migration; and within-site observations of water level on the bank, water depth, flow direction, flow velocity, and backwater effects are used to select a fishing location. In addition, the fishers described characteristics of habitat such as substrate, vegetation, and turbidity, as well as fish feeding habits and sequential migration patterns that represent valuable observations about fish ecology. The comparison with scientific information not only confirmed existing insights but also extended our understanding of ecohydrological relationships and helped explain possible causes of relevant long-term trends. In conclusion, our findings highlight the vital role of the flooding dynamics in the fishing practice and draw attention to the potential negative impacts of hydrologic alteration on the livelihoods of fishers.