The presence of particles in a flow can change the flow behaviour in an unpredictable manner, that leads us to multiphase flow. Multiphase flows are present in nature and in everyday life around us. The most common example of a multiphase flow, that one can think of, is the treatment of drinking water using liquid-solid fluidization in softening processes. Multiphase flows can also have very high relevance in various practical applications ranging from sedimentation of particles for process industries, transport of a dense suspension (slurry) through a pipe and dredging applications for land reclamation. Even though a lot of research has been done on liquid-solid fluidization in inertial and viscous dominated regimes, it remains far from being understood completely. Experimental studies were conducted on the fluidization of monodisperse solid spheres within a rectangular channel. This research saw the development of an experimental setup, to study this phenomenon. The bulk solid volume fraction was studied in three different regimes. At a dilute regime (Φ ≤ 4%), moderate regime (Φ = 8 − 14%), and a dense regime (Φ ≥ 15%). To study the impact of inertia, three distinct Galileo numbers in the inertial regime were examined: 𝐺𝑎 = 147, 196, and 252. At these values, a singular settling/rising sphere presents markedly varied wake and path characteristics. Throughout the study, the particle/fluid mass density ratio remained constant at 0.87. Results indicate that within the range of Φ = 4 − 18%, both the microstructure and dynamics of the suspension are heavily influenced by the bulk solid volume fraction. This aligns qualitatively with previous literature. Enhanced fluidization was noticed in the experiments. This is usually driven by the formation of clusters. Particle clustering within these regimes is driven by various mechanisms: trapping of particles in wakes, a drafting–kissing–tumbling (DKT) instability causing vertically aligned particles to swiftly realign horizontally, and multiparticle interactions with collisions playing a minor role. On the macrostructure level, intrinsic convection was captured in the fluidization column. To further study these characteristics, instantaneous snapshots were illustrated for varying cases. Profiles for both mean velocity and root-mean-square (RMS) velocity were obtained. Wall effects emerged as pronounced, diminishing in importance with increased bulk solid volume fraction and augmented 𝐺𝑎 values. Intrinsic convection is observed in these experiments, and the velocity profiles provided an indication. Probability density functions (pdf) for normalized velocities highlighted evident particle clustering.