FD
F.K. Dr. Kleiner
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Microalgal biorefineries offer a sustainable route to biological hydrogen production in C. reinhardtii, but are limited commercially by low achievable cell densities in liquid suspension culture. Encapsulating microalgae in alginate hydrogel beads can increase biomass per bioreactor volume, but how fabrication
technique, bead material properties, and algal growth relate to one another remains poorly characterized. This thesis uses in-air microfluidics (IAMF) to fabricate hydrogen-producing C. reinhardtii-laden alginate beads and links the resulting bead properties to algal growth. A viable material formulation space was identified, requiring high guluronate alginate at 0.5 w/v%, with sterilization method and crosslinking time strongly affecting precursor viscosity, bead formation parameters, and resulting cluster morphology and density. Over 11 days, cell density inside beads rose more than forty-fold, and total culture cell density was 68% higher than in liquid suspension controls, while beads also outperformed equivalent-volume bulk hydrogel constructs. Unanticipated bacterial contamination was identified as a key confounding variable. These results establish proof of concept that IAMF fabricated alginate beads can increase biomass density of C. reinhardtii relative to suspension culture and characterize their material properties, motivating future work to further optimize
and scale-up this concept. ...
technique, bead material properties, and algal growth relate to one another remains poorly characterized. This thesis uses in-air microfluidics (IAMF) to fabricate hydrogen-producing C. reinhardtii-laden alginate beads and links the resulting bead properties to algal growth. A viable material formulation space was identified, requiring high guluronate alginate at 0.5 w/v%, with sterilization method and crosslinking time strongly affecting precursor viscosity, bead formation parameters, and resulting cluster morphology and density. Over 11 days, cell density inside beads rose more than forty-fold, and total culture cell density was 68% higher than in liquid suspension controls, while beads also outperformed equivalent-volume bulk hydrogel constructs. Unanticipated bacterial contamination was identified as a key confounding variable. These results establish proof of concept that IAMF fabricated alginate beads can increase biomass density of C. reinhardtii relative to suspension culture and characterize their material properties, motivating future work to further optimize
and scale-up this concept. ...
Microalgal biorefineries offer a sustainable route to biological hydrogen production in C. reinhardtii, but are limited commercially by low achievable cell densities in liquid suspension culture. Encapsulating microalgae in alginate hydrogel beads can increase biomass per bioreactor volume, but how fabrication
technique, bead material properties, and algal growth relate to one another remains poorly characterized. This thesis uses in-air microfluidics (IAMF) to fabricate hydrogen-producing C. reinhardtii-laden alginate beads and links the resulting bead properties to algal growth. A viable material formulation space was identified, requiring high guluronate alginate at 0.5 w/v%, with sterilization method and crosslinking time strongly affecting precursor viscosity, bead formation parameters, and resulting cluster morphology and density. Over 11 days, cell density inside beads rose more than forty-fold, and total culture cell density was 68% higher than in liquid suspension controls, while beads also outperformed equivalent-volume bulk hydrogel constructs. Unanticipated bacterial contamination was identified as a key confounding variable. These results establish proof of concept that IAMF fabricated alginate beads can increase biomass density of C. reinhardtii relative to suspension culture and characterize their material properties, motivating future work to further optimize
and scale-up this concept.
technique, bead material properties, and algal growth relate to one another remains poorly characterized. This thesis uses in-air microfluidics (IAMF) to fabricate hydrogen-producing C. reinhardtii-laden alginate beads and links the resulting bead properties to algal growth. A viable material formulation space was identified, requiring high guluronate alginate at 0.5 w/v%, with sterilization method and crosslinking time strongly affecting precursor viscosity, bead formation parameters, and resulting cluster morphology and density. Over 11 days, cell density inside beads rose more than forty-fold, and total culture cell density was 68% higher than in liquid suspension controls, while beads also outperformed equivalent-volume bulk hydrogel constructs. Unanticipated bacterial contamination was identified as a key confounding variable. These results establish proof of concept that IAMF fabricated alginate beads can increase biomass density of C. reinhardtii relative to suspension culture and characterize their material properties, motivating future work to further optimize
and scale-up this concept.