GM
G.M.H. Meesters
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1
Chocolate milk, paint and snow globes are all examples of solid-liquid suspensions found in daily life. In industry, colloids are also commonly used to process solids, as liquids are typically easier to handle than powders. Given the environmental concerns surrounding the usage of organic solvents, which are often evaporated and released during the process, water-based colloids are preferred. However, not all particles have a natural affinity with water. When particles have insufficient affinity for water, the solid and liquid phases segregate. To enable such particles to still form stable water-based suspensions, their surface properties can be modified....
...
Chocolate milk, paint and snow globes are all examples of solid-liquid suspensions found in daily life. In industry, colloids are also commonly used to process solids, as liquids are typically easier to handle than powders. Given the environmental concerns surrounding the usage of organic solvents, which are often evaporated and released during the process, water-based colloids are preferred. However, not all particles have a natural affinity with water. When particles have insufficient affinity for water, the solid and liquid phases segregate. To enable such particles to still form stable water-based suspensions, their surface properties can be modified....
Granular flow in stirred bed reactors
Insights through radiation-based imaging techniques
Polypropylene (PP) is a versatile polymer extensively used in industries such as food packaging, automotive, healthcare, and textiles. Industrially, PP is produced via gas-phase solid-catalyzed polymerization in horizontal stirred bed, vertical stirred bed, or fluidized bed reactors. These reactors operate under controlled conditions to polymerize propylene monomers into solid PP particles. Despite their widespread application, operating these reactors is challenging due to a lack of fundamental understanding and modeling capabilities, which leads to reduced production capacity and lower quality of the final product. This gap in understanding is primarily due to the scarcity of detailed experimental data, which is difficult to obtain because of the opaqueness of the flow and the rapidly evolving gas-solids distribution, necessitating non-optical measurements with high temporal resolution.
In this dissertation, a deeper understanding of granular flow behavior in these reactors was achieved through detailed experimental measurements using radiation-based imaging. Recognizing the direct link between macro-scale flow behavior and particle-scale phenomena, this research spanned both scales. Although the primary focus of this thesis is on a horizontal stirred bed, experiments were also conducted using two additional lab-scale reactor configurations: a vertical stirred bed and a fluidized bed. High-quality data on flow patterns, phase holdup, and particle dynamics were obtained using X-ray imaging and single-photon emission radioactive particle tracking. A key novelty of this research was the use of industrial-grade powders, such as polypropylene reactor powder, as encountered in horizontal stirred bed reactors. The collected data were thoroughly analyzed to identify the key parameters influencing granular flow behavior, utilizing statistical methods and visualization tools to uncover critical insights.
First, the flow behavior of polypropylene reactor powder in a laboratoryscale horizontal stirred bed reactor (HSBR) was investigated using X-ray imaging. It was observed that agitation significantly dictates overall flow behavior and phase holdup in the HSBR. Gas injection through inlet points at the bottom resulted in spouting behavior, and the gas holdup at fixed agitator positions remained highly consistent across successive revolutions. The presence of liquid was found to deteriorate the flow behavior due to liquid bridging at particle contact points, with particle size and surface morphology influencing the powders’ susceptibility to liquid.
Subsequently, a single-photon emission radioactive particle tracking method was presented, allowing the tracking of individual photon-emitting particles to evaluate the hydrodynamics of multiphase flows. This method directly utilized detected photon hit locations to reconstruct the three-dimensional position of the tracer particle, avoiding assumptions in count rate fluctuations. The tracer particle’s position was determined by finding the intersection point of three two-dimensional planes from the detectors, achieving a spatial accuracy of approximately 1 mm through a subsequent calibration experimentation procedure.
Thereafter, the method was employed to characterize the particle dynamics in the HSBR. It was found that, besides the agitator rotation speed, the flow behavior is significantly influenced by the reactor fill level. At low rotation speeds and fill levels, solids motion was primarily induced by impeller blade passage, resulting in semi-static bed motion and poor solids distribution. Increased fill levels and rotation speeds led to continuous solids motion and uniform distribution. Solids circulation, quantified by a dimensionless cycle number, increased with higher fill levels and rotation speeds. The axial dispersion coefficient ranged from 10-6 to 10-5 m2 s-1, increasing with rotation speed, although no conclusive relationship with fill level was observed.
Thereafter, the fluidization behavior of Geldart B particles in a vertical stirred bed reactor was investigated using X-ray imaging, pressure drop measurements, and numerical simulations via Computational Fluid Dynamics (CFD) coupled with Discrete Element Method (DEM) and Immersed Boundary Method (IBM). The experimentally obtained minimum fluidization curve and time-averaged pressure drop showed good qualitative agreement with simulations. Visual observations indicated that increasing the agitator’s angular velocity reduced bubble size and improved bed homogeneity, as evidenced by reduced pressure fluctuations. Simulations revealed that while the impeller enhances solids agitation, a proper design study is essential, as static immersed bodies like the stirrer shaft can adversely impact solids motion.
Finally, the correlation between the fluidization behavior and flow properties of 10 commercially available cohesive powders was experimentally investigated. The fluidization quality of the powders in a laboratory-scale fluidized bed was assessed using a Fluidization Quality Index (FQI), computed by integrating gas holdup and its temporal variation acquired through X-ray imaging. Flowability was measured in a rotating drum operated at high speeds, which aerated the powder bed, a critical factor in correlating fluidization behavior with flow properties. This study established a positive correlation between cohesive powders’ flowability and fluidization quality, suggesting that fast and user-friendly flowability measurements in a rotating drum instrument can predict fluidization potential, aiding in process optimization and enhancing fluidization studies for cohesive powders.
In summary, the insights acquired from this thesis enhance the understanding of flow behavior and phase holdup in stirred bed reactors and cohesive fluidized beds. These findings can serve as a valuable foundation for designing, optimizing, and intensifying systems for the industrial-scale manufacturing of high-quality PP resins. ...
In this dissertation, a deeper understanding of granular flow behavior in these reactors was achieved through detailed experimental measurements using radiation-based imaging. Recognizing the direct link between macro-scale flow behavior and particle-scale phenomena, this research spanned both scales. Although the primary focus of this thesis is on a horizontal stirred bed, experiments were also conducted using two additional lab-scale reactor configurations: a vertical stirred bed and a fluidized bed. High-quality data on flow patterns, phase holdup, and particle dynamics were obtained using X-ray imaging and single-photon emission radioactive particle tracking. A key novelty of this research was the use of industrial-grade powders, such as polypropylene reactor powder, as encountered in horizontal stirred bed reactors. The collected data were thoroughly analyzed to identify the key parameters influencing granular flow behavior, utilizing statistical methods and visualization tools to uncover critical insights.
First, the flow behavior of polypropylene reactor powder in a laboratoryscale horizontal stirred bed reactor (HSBR) was investigated using X-ray imaging. It was observed that agitation significantly dictates overall flow behavior and phase holdup in the HSBR. Gas injection through inlet points at the bottom resulted in spouting behavior, and the gas holdup at fixed agitator positions remained highly consistent across successive revolutions. The presence of liquid was found to deteriorate the flow behavior due to liquid bridging at particle contact points, with particle size and surface morphology influencing the powders’ susceptibility to liquid.
Subsequently, a single-photon emission radioactive particle tracking method was presented, allowing the tracking of individual photon-emitting particles to evaluate the hydrodynamics of multiphase flows. This method directly utilized detected photon hit locations to reconstruct the three-dimensional position of the tracer particle, avoiding assumptions in count rate fluctuations. The tracer particle’s position was determined by finding the intersection point of three two-dimensional planes from the detectors, achieving a spatial accuracy of approximately 1 mm through a subsequent calibration experimentation procedure.
Thereafter, the method was employed to characterize the particle dynamics in the HSBR. It was found that, besides the agitator rotation speed, the flow behavior is significantly influenced by the reactor fill level. At low rotation speeds and fill levels, solids motion was primarily induced by impeller blade passage, resulting in semi-static bed motion and poor solids distribution. Increased fill levels and rotation speeds led to continuous solids motion and uniform distribution. Solids circulation, quantified by a dimensionless cycle number, increased with higher fill levels and rotation speeds. The axial dispersion coefficient ranged from 10-6 to 10-5 m2 s-1, increasing with rotation speed, although no conclusive relationship with fill level was observed.
Thereafter, the fluidization behavior of Geldart B particles in a vertical stirred bed reactor was investigated using X-ray imaging, pressure drop measurements, and numerical simulations via Computational Fluid Dynamics (CFD) coupled with Discrete Element Method (DEM) and Immersed Boundary Method (IBM). The experimentally obtained minimum fluidization curve and time-averaged pressure drop showed good qualitative agreement with simulations. Visual observations indicated that increasing the agitator’s angular velocity reduced bubble size and improved bed homogeneity, as evidenced by reduced pressure fluctuations. Simulations revealed that while the impeller enhances solids agitation, a proper design study is essential, as static immersed bodies like the stirrer shaft can adversely impact solids motion.
Finally, the correlation between the fluidization behavior and flow properties of 10 commercially available cohesive powders was experimentally investigated. The fluidization quality of the powders in a laboratory-scale fluidized bed was assessed using a Fluidization Quality Index (FQI), computed by integrating gas holdup and its temporal variation acquired through X-ray imaging. Flowability was measured in a rotating drum operated at high speeds, which aerated the powder bed, a critical factor in correlating fluidization behavior with flow properties. This study established a positive correlation between cohesive powders’ flowability and fluidization quality, suggesting that fast and user-friendly flowability measurements in a rotating drum instrument can predict fluidization potential, aiding in process optimization and enhancing fluidization studies for cohesive powders.
In summary, the insights acquired from this thesis enhance the understanding of flow behavior and phase holdup in stirred bed reactors and cohesive fluidized beds. These findings can serve as a valuable foundation for designing, optimizing, and intensifying systems for the industrial-scale manufacturing of high-quality PP resins. ...
Polypropylene (PP) is a versatile polymer extensively used in industries such as food packaging, automotive, healthcare, and textiles. Industrially, PP is produced via gas-phase solid-catalyzed polymerization in horizontal stirred bed, vertical stirred bed, or fluidized bed reactors. These reactors operate under controlled conditions to polymerize propylene monomers into solid PP particles. Despite their widespread application, operating these reactors is challenging due to a lack of fundamental understanding and modeling capabilities, which leads to reduced production capacity and lower quality of the final product. This gap in understanding is primarily due to the scarcity of detailed experimental data, which is difficult to obtain because of the opaqueness of the flow and the rapidly evolving gas-solids distribution, necessitating non-optical measurements with high temporal resolution.
In this dissertation, a deeper understanding of granular flow behavior in these reactors was achieved through detailed experimental measurements using radiation-based imaging. Recognizing the direct link between macro-scale flow behavior and particle-scale phenomena, this research spanned both scales. Although the primary focus of this thesis is on a horizontal stirred bed, experiments were also conducted using two additional lab-scale reactor configurations: a vertical stirred bed and a fluidized bed. High-quality data on flow patterns, phase holdup, and particle dynamics were obtained using X-ray imaging and single-photon emission radioactive particle tracking. A key novelty of this research was the use of industrial-grade powders, such as polypropylene reactor powder, as encountered in horizontal stirred bed reactors. The collected data were thoroughly analyzed to identify the key parameters influencing granular flow behavior, utilizing statistical methods and visualization tools to uncover critical insights.
First, the flow behavior of polypropylene reactor powder in a laboratoryscale horizontal stirred bed reactor (HSBR) was investigated using X-ray imaging. It was observed that agitation significantly dictates overall flow behavior and phase holdup in the HSBR. Gas injection through inlet points at the bottom resulted in spouting behavior, and the gas holdup at fixed agitator positions remained highly consistent across successive revolutions. The presence of liquid was found to deteriorate the flow behavior due to liquid bridging at particle contact points, with particle size and surface morphology influencing the powders’ susceptibility to liquid.
Subsequently, a single-photon emission radioactive particle tracking method was presented, allowing the tracking of individual photon-emitting particles to evaluate the hydrodynamics of multiphase flows. This method directly utilized detected photon hit locations to reconstruct the three-dimensional position of the tracer particle, avoiding assumptions in count rate fluctuations. The tracer particle’s position was determined by finding the intersection point of three two-dimensional planes from the detectors, achieving a spatial accuracy of approximately 1 mm through a subsequent calibration experimentation procedure.
Thereafter, the method was employed to characterize the particle dynamics in the HSBR. It was found that, besides the agitator rotation speed, the flow behavior is significantly influenced by the reactor fill level. At low rotation speeds and fill levels, solids motion was primarily induced by impeller blade passage, resulting in semi-static bed motion and poor solids distribution. Increased fill levels and rotation speeds led to continuous solids motion and uniform distribution. Solids circulation, quantified by a dimensionless cycle number, increased with higher fill levels and rotation speeds. The axial dispersion coefficient ranged from 10-6 to 10-5 m2 s-1, increasing with rotation speed, although no conclusive relationship with fill level was observed.
Thereafter, the fluidization behavior of Geldart B particles in a vertical stirred bed reactor was investigated using X-ray imaging, pressure drop measurements, and numerical simulations via Computational Fluid Dynamics (CFD) coupled with Discrete Element Method (DEM) and Immersed Boundary Method (IBM). The experimentally obtained minimum fluidization curve and time-averaged pressure drop showed good qualitative agreement with simulations. Visual observations indicated that increasing the agitator’s angular velocity reduced bubble size and improved bed homogeneity, as evidenced by reduced pressure fluctuations. Simulations revealed that while the impeller enhances solids agitation, a proper design study is essential, as static immersed bodies like the stirrer shaft can adversely impact solids motion.
Finally, the correlation between the fluidization behavior and flow properties of 10 commercially available cohesive powders was experimentally investigated. The fluidization quality of the powders in a laboratory-scale fluidized bed was assessed using a Fluidization Quality Index (FQI), computed by integrating gas holdup and its temporal variation acquired through X-ray imaging. Flowability was measured in a rotating drum operated at high speeds, which aerated the powder bed, a critical factor in correlating fluidization behavior with flow properties. This study established a positive correlation between cohesive powders’ flowability and fluidization quality, suggesting that fast and user-friendly flowability measurements in a rotating drum instrument can predict fluidization potential, aiding in process optimization and enhancing fluidization studies for cohesive powders.
In summary, the insights acquired from this thesis enhance the understanding of flow behavior and phase holdup in stirred bed reactors and cohesive fluidized beds. These findings can serve as a valuable foundation for designing, optimizing, and intensifying systems for the industrial-scale manufacturing of high-quality PP resins.
In this dissertation, a deeper understanding of granular flow behavior in these reactors was achieved through detailed experimental measurements using radiation-based imaging. Recognizing the direct link between macro-scale flow behavior and particle-scale phenomena, this research spanned both scales. Although the primary focus of this thesis is on a horizontal stirred bed, experiments were also conducted using two additional lab-scale reactor configurations: a vertical stirred bed and a fluidized bed. High-quality data on flow patterns, phase holdup, and particle dynamics were obtained using X-ray imaging and single-photon emission radioactive particle tracking. A key novelty of this research was the use of industrial-grade powders, such as polypropylene reactor powder, as encountered in horizontal stirred bed reactors. The collected data were thoroughly analyzed to identify the key parameters influencing granular flow behavior, utilizing statistical methods and visualization tools to uncover critical insights.
First, the flow behavior of polypropylene reactor powder in a laboratoryscale horizontal stirred bed reactor (HSBR) was investigated using X-ray imaging. It was observed that agitation significantly dictates overall flow behavior and phase holdup in the HSBR. Gas injection through inlet points at the bottom resulted in spouting behavior, and the gas holdup at fixed agitator positions remained highly consistent across successive revolutions. The presence of liquid was found to deteriorate the flow behavior due to liquid bridging at particle contact points, with particle size and surface morphology influencing the powders’ susceptibility to liquid.
Subsequently, a single-photon emission radioactive particle tracking method was presented, allowing the tracking of individual photon-emitting particles to evaluate the hydrodynamics of multiphase flows. This method directly utilized detected photon hit locations to reconstruct the three-dimensional position of the tracer particle, avoiding assumptions in count rate fluctuations. The tracer particle’s position was determined by finding the intersection point of three two-dimensional planes from the detectors, achieving a spatial accuracy of approximately 1 mm through a subsequent calibration experimentation procedure.
Thereafter, the method was employed to characterize the particle dynamics in the HSBR. It was found that, besides the agitator rotation speed, the flow behavior is significantly influenced by the reactor fill level. At low rotation speeds and fill levels, solids motion was primarily induced by impeller blade passage, resulting in semi-static bed motion and poor solids distribution. Increased fill levels and rotation speeds led to continuous solids motion and uniform distribution. Solids circulation, quantified by a dimensionless cycle number, increased with higher fill levels and rotation speeds. The axial dispersion coefficient ranged from 10-6 to 10-5 m2 s-1, increasing with rotation speed, although no conclusive relationship with fill level was observed.
Thereafter, the fluidization behavior of Geldart B particles in a vertical stirred bed reactor was investigated using X-ray imaging, pressure drop measurements, and numerical simulations via Computational Fluid Dynamics (CFD) coupled with Discrete Element Method (DEM) and Immersed Boundary Method (IBM). The experimentally obtained minimum fluidization curve and time-averaged pressure drop showed good qualitative agreement with simulations. Visual observations indicated that increasing the agitator’s angular velocity reduced bubble size and improved bed homogeneity, as evidenced by reduced pressure fluctuations. Simulations revealed that while the impeller enhances solids agitation, a proper design study is essential, as static immersed bodies like the stirrer shaft can adversely impact solids motion.
Finally, the correlation between the fluidization behavior and flow properties of 10 commercially available cohesive powders was experimentally investigated. The fluidization quality of the powders in a laboratory-scale fluidized bed was assessed using a Fluidization Quality Index (FQI), computed by integrating gas holdup and its temporal variation acquired through X-ray imaging. Flowability was measured in a rotating drum operated at high speeds, which aerated the powder bed, a critical factor in correlating fluidization behavior with flow properties. This study established a positive correlation between cohesive powders’ flowability and fluidization quality, suggesting that fast and user-friendly flowability measurements in a rotating drum instrument can predict fluidization potential, aiding in process optimization and enhancing fluidization studies for cohesive powders.
In summary, the insights acquired from this thesis enhance the understanding of flow behavior and phase holdup in stirred bed reactors and cohesive fluidized beds. These findings can serve as a valuable foundation for designing, optimizing, and intensifying systems for the industrial-scale manufacturing of high-quality PP resins.
Fermentation processes are considered to be essential to decrease our reliance on fossil fuel based products. However, the scale-up from lab-scale to industrial-scale has proven to be difficult. Computational Fluid Dynamics (CFD) has the potential to be a tool to optimize the scale-up and to help engineers understand the relevant hydrodynamics inside such reactors. However, traditional CFD simulations are computationally intensive and it is not uncommon that simulations can take several months to compute a few minutes of flow-time. Due to the recent development of GPU-based hardware, the Lattice-Boltzmann method (LBM) has been gaining much interest as this meant an orders-of-magnitude decrease of needed computational time compared to conventional CFD methods. In order to calibrate the models underlying the simulations, the obtained results of said simulations should be validated against experimentally obtained data, which is exceptionally scarce for industrial-scaled reactors. Hence the aim of this thesis is to to investigate the applicability of the LBM in high gas-flow, industrial sized reactors by studying three benchmark cases. Using Large Eddy Simulations (LES) and Euler-Lagrangian tracking of bubble parcels, the models provided by M-Star (MStar Simulations, LLC) are validated by replicating a small-scale reactor of which the LBM has already been successfully applied to. The industrial-scaled case consists of the simulation of the 22m3 industrial reactor located in Stavanger, which is an exception regarding the scarcity of experimental data for industrial sized reactors. As this data set did not encompass all the relevant data for aerated stirred tanks, the suitability of the LBM and provided models are also tested for a smaller scaled tank of which the Bubble Size Distribution (BSD) throughout the vessel is known. The applicability of the provided models will be tested by comparing the obtained gas hold-up, BSD and power consumption with experimental data sets. Although the LBM is suitable for industrial-scaled reactors, the provided models by M-Star are not sufficient to predict the gas hold-up and power consumption at high superficial gas velocities. The provided Free Particle drag correlation is not sufficient to describe the dispersion of bubbles throughout the vessel, leading to a significantly under-estimated gas hold-up. In addition, the parcel approach leads to the formation of large bubbles in the vessel. And although enforcing a maximum coalescence diameter does improve the BSD, the gas hold-up is not significantly influenced by the presence of large bubbles. Furthermore, the Euler-Lagrangian way of modeling bubble particles did not lead to the formation of gas cavities, which resulted in an insignificant drop in power consumption. Nevertheless, the current work provides a foundation for subsequent research.
...
Fermentation processes are considered to be essential to decrease our reliance on fossil fuel based products. However, the scale-up from lab-scale to industrial-scale has proven to be difficult. Computational Fluid Dynamics (CFD) has the potential to be a tool to optimize the scale-up and to help engineers understand the relevant hydrodynamics inside such reactors. However, traditional CFD simulations are computationally intensive and it is not uncommon that simulations can take several months to compute a few minutes of flow-time. Due to the recent development of GPU-based hardware, the Lattice-Boltzmann method (LBM) has been gaining much interest as this meant an orders-of-magnitude decrease of needed computational time compared to conventional CFD methods. In order to calibrate the models underlying the simulations, the obtained results of said simulations should be validated against experimentally obtained data, which is exceptionally scarce for industrial-scaled reactors. Hence the aim of this thesis is to to investigate the applicability of the LBM in high gas-flow, industrial sized reactors by studying three benchmark cases. Using Large Eddy Simulations (LES) and Euler-Lagrangian tracking of bubble parcels, the models provided by M-Star (MStar Simulations, LLC) are validated by replicating a small-scale reactor of which the LBM has already been successfully applied to. The industrial-scaled case consists of the simulation of the 22m3 industrial reactor located in Stavanger, which is an exception regarding the scarcity of experimental data for industrial sized reactors. As this data set did not encompass all the relevant data for aerated stirred tanks, the suitability of the LBM and provided models are also tested for a smaller scaled tank of which the Bubble Size Distribution (BSD) throughout the vessel is known. The applicability of the provided models will be tested by comparing the obtained gas hold-up, BSD and power consumption with experimental data sets. Although the LBM is suitable for industrial-scaled reactors, the provided models by M-Star are not sufficient to predict the gas hold-up and power consumption at high superficial gas velocities. The provided Free Particle drag correlation is not sufficient to describe the dispersion of bubbles throughout the vessel, leading to a significantly under-estimated gas hold-up. In addition, the parcel approach leads to the formation of large bubbles in the vessel. And although enforcing a maximum coalescence diameter does improve the BSD, the gas hold-up is not significantly influenced by the presence of large bubbles. Furthermore, the Euler-Lagrangian way of modeling bubble particles did not lead to the formation of gas cavities, which resulted in an insignificant drop in power consumption. Nevertheless, the current work provides a foundation for subsequent research.
Design of a treatment protocol to improve the health of B12 deficient patients
Diagnostic tools and corrective measures to analyse and improve cellular function of vitamin B12
The design project entitled “Design of a treatment protocol to improve the health of B12 deficient patients” is carried out as an individual design project of PDEng program in Chemical Product Design. This project is commenced for the B12 Institute, Rotterdam, which aims to improve the diagnosis and treatment of patients with vitamin B12 and folate deficiency. The experience of the B12 Institute showed that the current diagnostic and treatment protocol is not sufficient to improve the health of some severe patients.
This design project aims to provide knowledge beyond the classical theories of B12 deficiency, such that an extended protocol can be recommended to enhance the recovery and health of B12 deficient patients. The recommended protocol consists of diagnostic means and subsequent corrective actions.
The approach of the project is divided into 4 major steps:
1. study of the biochemistry of vitamin B12 cellular metabolism;
2. generation of hypotheses on disorders related to B12 deficiency according to the biochemistry study;
3. recommendation of biomarkers/diagnostic tools;
4. recommendation of corrective actions.
This report discusses the hypotheses of the vitamin B12 cellular inactivity, which extends the theory of the classical B12 deficiency. The hypotheses revolve around the failure of the B12 cellular activation due to enzymes defects and the activation cofactors deficiencies, as well as the failure of B12 co-enzymatic function due to oxidative stress. A series of vicious cycles between the overlooked causes and impacts in B12 inactivity is also described. The highlights of the hypotheses are summarized as follows:
• B12 deficiency causes folate cycle block, which leads to glycine deficiency,
• Glycine deficiency leads to glutathione deficiency and collagen deficiency,
• Glutathione deficiency causes an elevated oxidative stress, and vice versa,
• Collagen deficiency leads to intestinal bacterial dysbiosis,
• Intestinal bacterial dysbiosis causes the production of bacterial toxins, including formaldehyde,
• An excess of formaldehyde exacerbates oxidative stress and damages to the body, and may as well inactivate cellular B12.
However, we found that the current knowledge on the mechanisms explaining a lower B12 enzyme activity may still be insufficient to explain the whole condition and issues related to B12 cellular inactivity.
Finally, we designed a mini study to obtain evidence on a number of the hypotheses, especially those related to oxidative stress. New biomarkers for the extension of the diagnostic tools are explored. The new biomarkers are expected to provide better tools to explain the condition and symptoms of the patients. In addition, several supplements are recommended as corrective actions of the anticipated issues disclosed by the new biomarkers. The concepts and the results of the project are expected to provide new insights for the medical research and practice of B12 deficiency treatment, as well as to provide major improvement to the health of the patients.
...
This design project aims to provide knowledge beyond the classical theories of B12 deficiency, such that an extended protocol can be recommended to enhance the recovery and health of B12 deficient patients. The recommended protocol consists of diagnostic means and subsequent corrective actions.
The approach of the project is divided into 4 major steps:
1. study of the biochemistry of vitamin B12 cellular metabolism;
2. generation of hypotheses on disorders related to B12 deficiency according to the biochemistry study;
3. recommendation of biomarkers/diagnostic tools;
4. recommendation of corrective actions.
This report discusses the hypotheses of the vitamin B12 cellular inactivity, which extends the theory of the classical B12 deficiency. The hypotheses revolve around the failure of the B12 cellular activation due to enzymes defects and the activation cofactors deficiencies, as well as the failure of B12 co-enzymatic function due to oxidative stress. A series of vicious cycles between the overlooked causes and impacts in B12 inactivity is also described. The highlights of the hypotheses are summarized as follows:
• B12 deficiency causes folate cycle block, which leads to glycine deficiency,
• Glycine deficiency leads to glutathione deficiency and collagen deficiency,
• Glutathione deficiency causes an elevated oxidative stress, and vice versa,
• Collagen deficiency leads to intestinal bacterial dysbiosis,
• Intestinal bacterial dysbiosis causes the production of bacterial toxins, including formaldehyde,
• An excess of formaldehyde exacerbates oxidative stress and damages to the body, and may as well inactivate cellular B12.
However, we found that the current knowledge on the mechanisms explaining a lower B12 enzyme activity may still be insufficient to explain the whole condition and issues related to B12 cellular inactivity.
Finally, we designed a mini study to obtain evidence on a number of the hypotheses, especially those related to oxidative stress. New biomarkers for the extension of the diagnostic tools are explored. The new biomarkers are expected to provide better tools to explain the condition and symptoms of the patients. In addition, several supplements are recommended as corrective actions of the anticipated issues disclosed by the new biomarkers. The concepts and the results of the project are expected to provide new insights for the medical research and practice of B12 deficiency treatment, as well as to provide major improvement to the health of the patients.
...
The design project entitled “Design of a treatment protocol to improve the health of B12 deficient patients” is carried out as an individual design project of PDEng program in Chemical Product Design. This project is commenced for the B12 Institute, Rotterdam, which aims to improve the diagnosis and treatment of patients with vitamin B12 and folate deficiency. The experience of the B12 Institute showed that the current diagnostic and treatment protocol is not sufficient to improve the health of some severe patients.
This design project aims to provide knowledge beyond the classical theories of B12 deficiency, such that an extended protocol can be recommended to enhance the recovery and health of B12 deficient patients. The recommended protocol consists of diagnostic means and subsequent corrective actions.
The approach of the project is divided into 4 major steps:
1. study of the biochemistry of vitamin B12 cellular metabolism;
2. generation of hypotheses on disorders related to B12 deficiency according to the biochemistry study;
3. recommendation of biomarkers/diagnostic tools;
4. recommendation of corrective actions.
This report discusses the hypotheses of the vitamin B12 cellular inactivity, which extends the theory of the classical B12 deficiency. The hypotheses revolve around the failure of the B12 cellular activation due to enzymes defects and the activation cofactors deficiencies, as well as the failure of B12 co-enzymatic function due to oxidative stress. A series of vicious cycles between the overlooked causes and impacts in B12 inactivity is also described. The highlights of the hypotheses are summarized as follows:
• B12 deficiency causes folate cycle block, which leads to glycine deficiency,
• Glycine deficiency leads to glutathione deficiency and collagen deficiency,
• Glutathione deficiency causes an elevated oxidative stress, and vice versa,
• Collagen deficiency leads to intestinal bacterial dysbiosis,
• Intestinal bacterial dysbiosis causes the production of bacterial toxins, including formaldehyde,
• An excess of formaldehyde exacerbates oxidative stress and damages to the body, and may as well inactivate cellular B12.
However, we found that the current knowledge on the mechanisms explaining a lower B12 enzyme activity may still be insufficient to explain the whole condition and issues related to B12 cellular inactivity.
Finally, we designed a mini study to obtain evidence on a number of the hypotheses, especially those related to oxidative stress. New biomarkers for the extension of the diagnostic tools are explored. The new biomarkers are expected to provide better tools to explain the condition and symptoms of the patients. In addition, several supplements are recommended as corrective actions of the anticipated issues disclosed by the new biomarkers. The concepts and the results of the project are expected to provide new insights for the medical research and practice of B12 deficiency treatment, as well as to provide major improvement to the health of the patients.
This design project aims to provide knowledge beyond the classical theories of B12 deficiency, such that an extended protocol can be recommended to enhance the recovery and health of B12 deficient patients. The recommended protocol consists of diagnostic means and subsequent corrective actions.
The approach of the project is divided into 4 major steps:
1. study of the biochemistry of vitamin B12 cellular metabolism;
2. generation of hypotheses on disorders related to B12 deficiency according to the biochemistry study;
3. recommendation of biomarkers/diagnostic tools;
4. recommendation of corrective actions.
This report discusses the hypotheses of the vitamin B12 cellular inactivity, which extends the theory of the classical B12 deficiency. The hypotheses revolve around the failure of the B12 cellular activation due to enzymes defects and the activation cofactors deficiencies, as well as the failure of B12 co-enzymatic function due to oxidative stress. A series of vicious cycles between the overlooked causes and impacts in B12 inactivity is also described. The highlights of the hypotheses are summarized as follows:
• B12 deficiency causes folate cycle block, which leads to glycine deficiency,
• Glycine deficiency leads to glutathione deficiency and collagen deficiency,
• Glutathione deficiency causes an elevated oxidative stress, and vice versa,
• Collagen deficiency leads to intestinal bacterial dysbiosis,
• Intestinal bacterial dysbiosis causes the production of bacterial toxins, including formaldehyde,
• An excess of formaldehyde exacerbates oxidative stress and damages to the body, and may as well inactivate cellular B12.
However, we found that the current knowledge on the mechanisms explaining a lower B12 enzyme activity may still be insufficient to explain the whole condition and issues related to B12 cellular inactivity.
Finally, we designed a mini study to obtain evidence on a number of the hypotheses, especially those related to oxidative stress. New biomarkers for the extension of the diagnostic tools are explored. The new biomarkers are expected to provide better tools to explain the condition and symptoms of the patients. In addition, several supplements are recommended as corrective actions of the anticipated issues disclosed by the new biomarkers. The concepts and the results of the project are expected to provide new insights for the medical research and practice of B12 deficiency treatment, as well as to provide major improvement to the health of the patients.