Template-free 2D particle fusion in localization microscopy

Journal article (2018)

Authors

H. Heydarian ImPhys/Quantitative Imaging
Florian Schueder Ludwig Maximilians University, Max Planck Institute of Biochemistry
Maximilian T. Strauss Max Planck Institute of Biochemistry, Ludwig Maximilians University
Ben van Werkhoven Netherlands eScience Center
Mohamadreza Fazel University of New Mexico
K.A. Lidke University of New Mexico
Ralf Jungmann Max Planck Institute of Biochemistry, Ludwig Maximilians University
S. Stallinga ImPhys/Imaging Physics
B. Rieger ImPhys/Quantitative Imaging
Research Group
ImPhys/Quantitative Imaging
Copyright: © 2018 H. Heydarian, Florian Schueder, Maximilian T. Strauss, Ben van Werkhoven, Mohamadreza Fazel, K.A. Lidke, Ralf Jungmann, S. Stallinga, B. Rieger
DOI: https://doi.org/10.1038/s41592-018-0136-6
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Published Date

2018

Language

English

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Research Group

ImPhys/Quantitative Imaging

Abstract

Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.

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