Template-free 2D particle fusion in localization microscopy
Hamidreza Heydarian (TU Delft - ImPhys/Quantitative Imaging)
Florian Schueder (Ludwig Maximilians University, Max Planck Institute of Biochemistry)
Maximilian T. Strauss (Ludwig Maximilians University, Max Planck Institute of Biochemistry)
Ben van Werkhoven (Netherlands eScience Center)
Mohamadreza Fazel (University of New Mexico)
Keith A. Lidke (University of New Mexico)
Ralf Jungmann (Max Planck Institute of Biochemistry, Ludwig Maximilians University)
Sjoerd Stallinga (TU Delft - ImPhys/Imaging Physics)
Bernd Rieger (TU Delft - ImPhys/Quantitative Imaging)
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Abstract
Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against individual misregistrations and underlabeling. We achieved 3.3-nm Fourier ring correlation (FRC) image resolution by fusing 383 DNA origami nanostructures with 80% labeling density, and 5.0-nm resolution for structures with 30% labeling density.