Diattenuation of brain tissue and its impact on 3D polarized light imaging

Journal article (2017)

Authors

Miriam Menzel Forschungszentrum Jülich
Julia Reckfort Forschungszentrum Jülich
D.J. Weigand Rheinisch-Westfälische Technische Hochschule
Hasan Köse Forschungszentrum Jülich
Katrin Amunts Universität Düsseldorf, Forschungszentrum Jülich
Markus Axer Forschungszentrum Jülich
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Published Date

2017

Language

English

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Abstract

3D-polarized light imaging (3D-PLI) reconstructs nerve fibers in histological brain sections by measuring their birefringence. This study investigates another effect caused by the optical anisotropy of brain tissue – diattenuation. Based on numerical and experimental studies and a complete analytical description of the optical system, the diattenuation was determined to be below 4 % in rat brain tissue. It was demonstrated that the diattenuation effect has negligible impact on the fiber orientations derived by 3D-PLI. The diattenuation signal, however, was found to highlight different anatomical structures that cannot be distinguished with current imaging techniques, which makes Diattenuation Imaging a promising extension to 3D-PLI.