Magnetospirillum sulfuroxidans sp. nov., capable of sulfur-dependent lithoautotrophy and a taxonomic reevaluation of the order Rhodospirillales

Journal article (2023)

Authors

Veronika V. Koziaeva Russian Academy of Sciences
Dimitry Y. Sorokin BT/Environmental Biotechnology - AS , Russian Academy of Sciences
Tatyana V. Kolganova Russian Academy of Sciences
Denis S. Grouzdev SciBear OU, Tallinn
Research Group
BT/Environmental Biotechnology (AS) (TU Delft)
Copyright: © 2023 Veronika V. Koziaeva, Dimitry Y. Sorokin, Tatiana V. Kolganova, Denis S. Grouzdev
DOI
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https://doi.org/10.1016/j.syapm.2023.126406
Magnetospirillum Paramagnetospirillum Magnetospirillaceae Rhodospirillaceae Rhodospirillales Magnetotactic bacteria
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Published Date

2023

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Environmental Biotechnology

Abstract

A spiral-shaped, highly motile bacterium was isolated from freshwater sulfidic sediment. Strain J10
T is a facultative autotroph utilizing sulfide, thiosulfate, and sulfur as the electron donors in microoxic conditions. Despite high 16S rRNA gene sequence sequence identity to Magnetospirillum gryphiswaldense MSR-1
T (99.6 %), digital DNA-DNA hybridisation homology and average nucleotide identity between the two strains was of the different species level (25 % and 83 %, respectively). Strain J10
T is not magnetotactic. The DNA G + C content of strain J10
T is 61.9 %. The predominant phospholipid ester-linked fatty acids are C18:1ω7, C16:1ω7, and C16:0. Strain J10
T (=DSM 23205
T = VKM B-3486
T) is the first strain of the genus Magnetospirillum showing lithoautotrophic growth and is proposed here as a novel species, Magnetospirillum sulfuroxidans sp. nov. In addition, we propose to establish a framework for distinguishing genera and families within the order Rhodospirillales based on phylogenomic analysis using the threshold values for average amino acid identity at ̴ 72 % for genera and ̴ 60 % for families. According to this, we propose to divide the existing genus Magnetospirillum into three genera: Magnetospirillum, Paramagnetospirillum, and Phaeospirillum, constituting a separate family Magnetospirillaceae fam. nov. in the order Rhodospirillales. Furthermore, phylogenomic data suggest that this order should accomodate six more new family level groups including Magnetospiraceae fam. nov., Magnetovibrionaceae fam. nov., Dongiaceae fam. nov., Niveispirillaceae fam. nov., Fodinicurvataceae fam. nov., and Oceanibaculaceae fam. nov.