3D Tracking of Motile Microorganisms
An Experimental Investigation of the Kinematics and Interactions of C. Reinhardtii
J. Mehmood (TU Delft - Mechanical Engineering)
D.S.W. Tam – Promotor (TU Delft - Mechanical Engineering)
A.J.L.L. Buchner – Copromotor (TU Delft - Mechanical Engineering)
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Abstract
This thesis presents a novel 3D tracking system for studying microorganism locomotion in complex fluid environments, addressing the limitations of current 2D and 3D techniques . The multi-camera microscopy system developed allows for high-resolution tracking of microswimmers like Chlamydomonas reinhardtii, providing new insights into their helical swimming patterns and interactions. The work includes the study of pairwise interactions and the impact of non-Newtonian fluids on motility, revealing key differences in swimming behavior and fluid dynamics in different media type.