Self-orienting hydrogel micro-buckets as novel cell carriers
Qian Liu (TU Delft - Applied Sciences, Beijing Normal University)
Meng Zhao (TU Delft - (OLD) MSE-1)
Serhii Mytnyk (TU Delft - Applied Sciences)
Benjamin Klemm (TU Delft - Applied Sciences)
Kai Zhang (TU Delft - Applied Sciences)
Yiming Wang (TU Delft - Applied Sciences)
Dadong Yan (Beijing Normal University)
Eduardo Mendes (TU Delft - Applied Sciences)
Jan H. van Esch (TU Delft - Applied Sciences)
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Abstract
Hydrogel microparticles are important in materials engineering, but their applications remain limited owing to the difficulties associated with their manipulation. Herein, we report the self-orientation of crescent-shaped hydrogel microparticles and elucidate its mechanism. Additionally, the microparticles were used, for the first time, as micro-buckets to carry living cells. In aqueous solution, the microparticles spontaneously rotated to a preferred orientation with the cavity facing up. We developed a geometric model that explains the self-orienting behavior of crescent-shaped particles by minimizing the potential energy of this specific morphology. Finally, we selectively modified the particles’ cavities with RGD peptide and exploited their preferred orientation to load them with living cells. Cells could adhere, proliferate, and be transported and released in vitro. These micro-buckets hold a great potential for applications in smart materials, cell therapy, and biological engineering.
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