A 3D microelectrode array to record neural activity at different tissue depths

Poster (2020)

Authors

T.M. de Rijk Student
Michel Hu Leiden University Medical Center
Jean-Philippe Frimat Leiden University Medical Center
Arn M.J.M. van den Maagdenberg Leiden University Medical Center
Pasqualina M Sarro Electronic Components, Technology and Materials - EEMCS
Massimo Mastrangeli Electronic Components, Technology and Materials - EEMCS
Research Group
Electronic Components, Technology and Materials (EEMCS) (TU Delft)
Copyright: © 2020 T.M. de Rijk, Michel Hu, Jean-Philippe Frimat, Arn M.J.M. van den Maagdenberg, Pasqualina M Sarro, Massimo Mastrangeli
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Published Date

2020

Language

English

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Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Microelectronics

Research Group

Electronic Components, Technology and Materials

Abstract

In vitro study of high-level neurobiological systems requires three-dimensional (3D) neuronal cultures [1]. Meas-uring responses along all three spatial dimensions is critical to record electric activity inside 3D neuronal models, such as organoids and other 3D brain tissue constructs. However, this lies beyond the capacity of 2D microelec-trode arrays (MEAs) [2]. We present planar arrays of 3D micro-pyramids, whereby each micro-pyramid supports multiple, electrically distinct and vertically stacked microelectrodes. The 3D microarrays were produced by wafer-scale micromachining and assembled onto printed circuit boards (PCBs) conforming to MEA readout standards.