Oleate hydratase catalyzes the hydration of a nonactivated carbon-carbon bond

Journal Article (2009)
Author(s)

Loes E. Bevers

M. W H Pinkse (TU Delft - OLD BT/Analytical Biotechnology)

P. D E M Verhaert (TU Delft - Applied Sciences)

Wilfred R. Hagen (TU Delft - Applied Sciences)

Research Group
BT/Biocatalysis
DOI related publication
https://doi.org/10.1128/JB.00306-09 Final published version
More Info
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Publication Year
2009
Language
English
Research Group
BT/Biocatalysis
Issue number
15
Volume number
191
Pages (from-to)
5010-5012
Downloads counter
66

Abstract

The hydration of oleic acid into 10-hydroxystearic acid was originally described for a Pseudomonas cell extract almost half a century ago. In the intervening years, the enzyme has never been characterized in any detail. We report here the isolation and characterization of oleate hydratase (EC 4.2.1.53) from Elizabethkingia meningoseptica.