Oleate hydratase catalyzes the hydration of a nonactivated carbon-carbon bond
Loes E. Bevers
M. W H Pinkse (TU Delft - OLD BT/Analytical Biotechnology)
P. D E M Verhaert (TU Delft - BT/Afdelingsbureau)
Wilfred R. Hagen (TU Delft - BT/Biocatalysis)
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Abstract
The hydration of oleic acid into 10-hydroxystearic acid was originally described for a Pseudomonas cell extract almost half a century ago. In the intervening years, the enzyme has never been characterized in any detail. We report here the isolation and characterization of oleate hydratase (EC 4.2.1.53) from Elizabethkingia meningoseptica.
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