Kinetics of DNA looping by Anabaena sensory rhodopsin transducer (ASRT) by using DNA cyclization assay

Journal article (2021)

Authors

Jaejin Lee Sogang University
S.H. Kim BN/Chirlmin Joo Lab - AS , Kavli institute of nanoscience Delft
Keon Ah Lee Sogang University
Kimleng Chuon Sogang University
Kwang Hwan Jung Sogang University
Doseok Kim Sogang University
Research Group
BN/Chirlmin Joo Lab (AS) (TU Delft)
Copyright: © 2021 Jae Jin Lee, S.H. Kim, Keon Ah Lee, Kimleng Chuon, Kwang Hwan Jung, Doseok Kim
DOI
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https://doi.org/10.1038/s41598-021-03148-4
More Info
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Published Date

2021

Language

English

Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Chirlmin Joo Lab

Abstract

DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for the optimal dye-labeling. This assay was applied to study how Anabaena sensory rhodopsin transducer (ASRT) facilitates loop formation of DNA as a possible mechanism for gene regulation. The ASRT-induced DNA looping was maximized at 50 mM of Na+, while Mg2+ also played an essential role in the loop formation.