Continuous infusion of manganese improves contrast and reduces side effects in manganese-enhanced magnetic resonance imaging studies

Journal article (2017)

Authors

Dana S. Poole Leiden University Medical Center
Nathalie Doorenweerd Leiden University Medical Center
Jaap J. Plomp Leiden University Medical Center
A.M.E.T.A. Mahfouz Pattern Recognition and Bioinformatics - EEMCS
M.J.T. Reinders Pattern Recognition and Bioinformatics - EEMCS
Louise van der Weerd Leiden University Medical Center
Research Group
Pattern Recognition and Bioinformatics (EEMCS) (TU Delft)
MEMRI Manganese MRI Side effects Manganese toxicity Longitudinal monitoring
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Published Date

2017

Language

English

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Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Intelligent Systems

Research Group

Pattern Recognition and Bioinformatics

Abstract

The ability to administer systemically high doses of manganese as contrast agent while circumventing its toxicity is of particular interest for exploratory MRI studies of the brain. Administering low doses either repeatedly or continuously over time has been shown to enable the acquisition of satisfactory MRI images of the mouse brain without apparent side effects. Here we have systematically compared the obtained MRI contrast and recorded potential systemic side effects such as stress response and muscle strength impairment in relation to the
achieved contrast. We show in mice that administering MnCl2 via osmotic infusion pumps allows for a sideeffect free delivery of a high cumulative dose of manganese chloride (480 mg/kg bodyweight in 8 days). High contrast in MRI was achieved while we did not observe the weight loss or distress seen in other studies where mice received manganese via fractionated intraperitoneal injections of lower doses of manganese. As the normal daily conduct of the mice was not affected, this new manganese delivery method might be of particular use to study brain activity over several days. This may facilitate the phenotyping of new transgenic mouse models, the study of chronic disease models and the monitoring of changes in brain activity in long-term behavioral studies.