Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells

Journal Article (2020)

Authors

Chris S. Vink (The University of Edinburgh)

Fernando Jose Calero-Nieto (University of Cambridge)

Xiaonan Wang (University of Cambridge)

Antonio Maglitto (The University of Edinburgh)

Samanta A. Mariani (The University of Edinburgh)

Wajid Jawaid (University of Cambridge)

Berthold Göttgens (University of Cambridge)

Elaine Dzierzak (The University of Edinburgh)

Affiliation

External organisation

Development Heterogeneity Transplantation Embryo AGM Hematopoietic stem cell Gata2 CD27 Functional identity Intra-aortic hematopoietic clusters Single-cell transcriptome

To reference this document use:

https://doi.org/10.1016/j.celrep.2020.107627

TU Delft Repository resolver:

https://resolver.tudelft.nl/773f91a7-47ee-4c23-8fbb-56d259da8ec9

More Info

expand_more

Publication Year

2020

Language

English

Affiliation

External organisation

Issue number

Volume number

DOI:

https://doi.org/10.1016/j.celrep.2020.107627

Abstract

Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.

No files available

Metadata only record. There are no files for this record.