Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells

Journal article (2020)

Authors

C.S. Vink The University of Edinburgh
Fernando Jose Calero-Nieto University of Cambridge
Xiaonan Wang University of Cambridge
Antonio Maglitto The University of Edinburgh
Samanta A. Mariani The University of Edinburgh
Wajid Jawaid University of Cambridge
Berthold Göttgens University of Cambridge
Elaine Dzierzak The University of Edinburgh
Affiliation
External organisation
Development Heterogeneity Transplantation Embryo AGM Hematopoietic stem cell Gata2 CD27 Functional identity Intra-aortic hematopoietic clusters Single-cell transcriptome
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Published Date

2020

Language

English

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Affiliation

External organisation

Abstract

Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.