Radar interferoinetry can be used to obtain sub-kilometer resolution over a swath at the expense of additional transmit power and a sufficiently long baseline to accommodate at least two antennas. This paper reports an innovative concept called AltiCube+, a low-cost long fixed-baseline interferometric radar altimeter based on CubeSats on-orbit assembly. The AltiCube+ concept consists of multiple 16U CubeSats, After an early operation and commissioning phase, these CubeSats will perform autonomous rendezvous and docking with each other via deployable booms to establish a long fixed-baseline, and then deploy antennas for an interferometric altimeter configuration. The uniqueness of AltiCube+ is on the potential scientific opportunities brought by two left and right looking interferometric altimeters with around 6 meter baseline (total system length is more than 8 m) and the sustainability due to its significantly low cost and short development lifecycle. If budget allows, multiple AltiCube+ systems with same or different altimetry capabilities can form a constellation to dramatically reduce the revisit time and, therefore, provide much better spatiotemporal coverage.

Vink and colleagues capture the transcriptome of the first functional HSCs in mouse by single-cell RNA-seq, index-sorting, and in vivo and invitro hematopoietic analyses. The HSC transcriptome is unique compared to HPCs, and heterogeneous expression of pivotal genes suggests that establishment of functional hematopoietic fate during cell emergence from embryonic aortic endothelium is stochastic.

Hematopoietic stem cells (HSCs) develop from the hemogenic endothelium in cluster structures that protrude into the embryonic aortic lumen. Although much is known about the molecular characteristics of the developing hematopoietic cells, we lack a complete understanding of their origin and the three-dimensional organization of the niche. Here, we use advanced live imaging techniques of organotypic slice cultures, clonal analysis, and mathematical modeling to show the two-step process of intra-aortic hematopoietic cluster (IACH) formation. First, a hemogenic progenitor buds up from the endothelium and undergoes division forming the monoclonal core of the IAHC. Next, surrounding hemogenic cells are recruited into the IAHC, increasing their size and heterogeneity. We identified the Notch ligand Dll4 as a negative regulator of the recruitment phase of IAHC. Blocking of Dll4 promotes the entrance of new hemogenic Gfi1⁺ cells into the IAHC and increases the number of cells that acquire HSC activity. Mathematical modeling based on our data provides estimation of the cluster lifetime and the average recruitment time of hemogenic cells to the cluster under physiologic and Dll4-inhibited conditions.