A Bienzymatic Cascade for the Complete Hydrolysis of Phthalic Acid Esters
Liu Yang (South China University of Technology)
Yunjian Ma (South China University of Technology, Macau University of Science and Technology)
Yebao Chen (South China University of Technology)
F Hollmann (TU Delft - BT/Biocatalysis)
Yonghua Wang (GuangdongYoumeiInstituteof IntelligentBio-manufacturing, Guangdong)
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Abstract
Phthalic acid esters (PAEs) are widely used as plastic additives to increase the flexibility and durability of plastics. Constantly leaching out from plastics, PAEs are ubiquitously found in the environment. As PAEs exhibit biological activities such as being endocrine disruptive, the quest for efficient degradation strategies continues. Here, we report a bienzymatic degradation system for PAEs to phthalic acid (PA) using a cascade comprising two hydrolases, EstJ6 and P8219. The reaction conditions were optimized with respect to concentrations of both enzymes, temperature, and initial pH. Finally, the substrate scope of the new cascade was investigated, revealing that particularly PAEs with relatively small alcohols were degraded to more than 90 %. This present study provides a potential doable biocatalytic strategy for the complete hydrolysis of PAEs.