The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus
Jordi F. Pelkmans (Universiteit Utrecht)
Aurin Vos (Universiteit Utrecht)
Karin Scholtmeijer (Universiteit Utrecht)
Ed Hendrix (Wageningen University & Research)
Johan J.P. Baars (Wageningen University & Research)
T Gehrmann (TU Delft - Pattern Recognition and Bioinformatics)
M.J.T. Reinders (TU Delft - Pattern Recognition and Bioinformatics)
Luis G. Lugones (Universiteit Utrecht)
Han A.B. Wösten (Universiteit Utrecht)
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Abstract
The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.
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