The transcriptional regulator c2h2 acceleratesmushroom formation in Agaricus bisporus

Journal article (2016)

Authors

Jordi F. Pelkmans Universiteit Utrecht
A.M. Vos Universiteit Utrecht
Karin Scholtmeijer Universiteit Utrecht
Ed Hendrix Wageningen University & Research
Johan J.P. Baars Wageningen University & Research
T. Gehrmann Pattern Recognition and Bioinformatics - EEMCS
M.J.T. Reinders Pattern Recognition and Bioinformatics - EEMCS
Luis G. Lugones Universiteit Utrecht
Han A.B. Wösten Universiteit Utrecht
Research Group
Pattern Recognition and Bioinformatics (EEMCS) (TU Delft)
Copyright: © 2016 Jordi F. Pelkmans, A.M. Vos, Karin Scholtmeijer, Ed Hendrix, Johan J.P. Baars, T. Gehrmann, M.J.T. Reinders, Luis G. Lugones, Han A.B. Wösten
DOI: https://doi.org/10.1007/s00253-016-7574-9
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Published Date

2016

Language

English

Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Intelligent Systems

Research Group

Pattern Recognition and Bioinformatics

Abstract

The Cys2His2 zinc finger protein gene c2h2 of Schizophyllum commune is involved in mushroom formation. Its inactivation results in a strain that is arrested at the stage of aggregate formation. In this study, the c2h2 orthologue of Agaricus bisporus was over-expressed in this white button mushroom forming basidiomycete using Agrobacterium-mediated transformation. Morphology, cap expansion rate, and total number and biomass of mushrooms were not affected by over-expression of c2h2. However, yield per day of the c2h2 over-expression strains peaked 1 day earlier. These data and expression analysis indicate that C2H2 impacts timing of mushroom formation at an early stage of development, making its encoding gene a target for breeding of commercial mushroom strains.