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Spatial analysis of transcript and protein levels in skeletal muscle

Journal Article (2024)
Author(s)

Paola Pisterzi (The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center)

Clara Martinez Mir (The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center)

Ouafa Dahri (Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW))

Isabel de Poorter (Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW))

Sandra Batlles Parera (Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW))

Milica Dostanić (Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW))

Massimo Mastrangeli (TU Delft - Electronic Components, Technology and Materials)

Christine Mummery (Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW))

Niels Geijsen (Leiden University Medical Center, The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW))

Fanny Sage (The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center)

Research Group
Electronic Components, Technology and Materials
DOI related publication
https://doi.org/10.1016/j.xpro.2024.103378
More Info
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Publication Year
2024
Language
English
Research Group
Electronic Components, Technology and Materials
Issue number
4
Volume number
5
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Abstract

Skeletal muscle spatial analyses have revealed unexpected regionalized gene expression patterns challenging the understanding of muscle as a homogeneous tissue. Here, we present a protocol for the spatial analysis of transcript and protein levels in murine skeletal muscle. We describe steps for tibialis anterior dissection, formaldehyde fixation, tissue chopper cutting, and hybridization chain reaction (HCR) detection and amplification. We then detail procedures for immunostaining, tissue clearing, and imaging. This protocol is easily adaptable to other tissues.