Studying genomic processes at the single-molecule level

introducing the tools and applications

Journal Article (2013)
Author(s)

D. Dulin (TU Delft - BN/Nynke Dekker Lab)

Jan Lipfert (TU Delft - BN/Nynke Dekker Lab)

M.C. Moolman (TU Delft - BN/Nynke Dekker Lab)

Nynke Dekker (TU Delft - BN/Nynke Dekker Lab)

Research Group
BN/Nynke Dekker Lab
Copyright
© 2013 D. Dulin, J. Lipfert, M.C. Moolman, N.H. Dekker
DOI related publication
https://doi.org/10.1038/nrg3316
More Info
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Publication Year
2013
Language
English
Copyright
© 2013 D. Dulin, J. Lipfert, M.C. Moolman, N.H. Dekker
Research Group
BN/Nynke Dekker Lab
Issue number
1
Volume number
14
Pages (from-to)
9-22
Reuse Rights

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Abstract

To understand genomic processes such as transcription, translation or splicing, we need to be able to study their spatial and temporal organization at the molecular level. Single-molecule approaches provide this opportunity, allowing researchers to monitor molecular conformations, interactions or diffusion quantitatively and in real time in purified systems and in the context of the living cell. This Review introduces the types of application of single-molecule approaches that can enhance our understanding of genome function.

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