High-Speed Super-Resolution Imaging Using Protein-Assisted DNA-PAINT
Mike Filius (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
Tao Ju Cui (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
Adithya N. Ananth (Kavli institute of nanoscience Delft, TNO, TU Delft - BN/Cees Dekker Lab)
Margreet W. Docter (TU Delft - BN/Technici en Analisten, Kavli institute of nanoscience Delft)
Jorrit W. Hegge (Wageningen University & Research)
John van der Oost (Wageningen University & Research)
Chirlmin Joo (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
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Abstract
Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), which allows for faster binding. Ago preorders the DNA imager strand into a helical conformation, allowing for 10 times faster target binding. Using a 2D DNA origami structure, we demonstrate that Ago-assisted DNA-PAINT (Ago-PAINT) can speed up the current DNA-PAINT technique by an order of magnitude, while maintaining the high spatial resolution. We envision this tool to be useful for super-resolution imaging and other techniques that rely on nucleic acid interactions.
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