Alcohol Dehydrogenases Catalyze the Reduction of Thioesters

Journal Article (2017)
Author(s)

Sabry H.H. Younes (Sohag University, TU Delft - BT/Biocatalysis)

Y. Ni (TU Delft - BT/Biocatalysis)

S. Schmidt (TU Delft - BT/Biocatalysis)

Wolfgang Kroutil (Graz University of Technology)

F Hollmann (TU Delft - BT/Biocatalysis)

Research Group
BT/Biocatalysis
Copyright
© 2017 S.H.H. Younes, Y. Ni, S. Schmidt, Wolfgang Kroutil, F. Hollmann
DOI related publication
https://doi.org/10.1002/cctc.201700165
More Info
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Publication Year
2017
Language
English
Copyright
© 2017 S.H.H. Younes, Y. Ni, S. Schmidt, Wolfgang Kroutil, F. Hollmann
Research Group
BT/Biocatalysis
Issue number
8
Volume number
9
Pages (from-to)
1389-1392
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Abstract

Alcohol dehydrogenases are well-established catalysts for various reduction reactions. However, the reduction of carboxylic acid derivatives has not yet been reported with these enzymes. In this contribution, we demonstrated that carboxylic acid thioesters could be readily reduced by a range of alcohol dehydrogenases, albeit at significantly reduced rates relative to those observed for corresponding ketones. A molecular explanation, especially for the lower turnover rates for thioesters relative to those obtained for ketones, is presented, as is a preliminary substrate scope.

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