Completing the canvas
advances and challenges for DNA-PAINT super-resolution imaging
R.G. van Wee (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
Mike Filius (TU Delft - BN/Chirlmin Joo Lab, Kavli institute of nanoscience Delft)
Chirlmin Joo (Kavli institute of nanoscience Delft, TU Delft - BN/Chirlmin Joo Lab)
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Abstract
Single-molecule localization microscopy (SMLM) is a potent tool to examine biological systems with unprecedented resolution, enabling the investigation of increasingly smaller structures. At the forefront of these developments is DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT), which exploits the stochastic and transient binding of fluorescently labeled DNA probes. In its early stages the implementation of DNA-PAINT was burdened by low-throughput, excessive acquisition time, and difficult integration with live-cell imaging. However, recent advances are addressing these challenges and expanding the range of applications of DNA-PAINT. We review the current state of the art of DNA-PAINT in light of these advances and contemplate what further developments remain indispensable to realize live-cell imaging.
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