Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays
Nele Revyn (TU Delft - Electrical Engineering, Mathematics and Computer Science)
Michel H.Y. Hu (Leiden University Medical Center)
Jean-Philippe Frimat (Leiden University Medical Center)
Bjorn de Wagenaar (TU Delft - Electrical Engineering, Mathematics and Computer Science)
Arn M.J.M. van den Maagdenberg (Leiden University Medical Center)
Pasqualina M. Sarro (TU Delft - Electrical Engineering, Mathematics and Computer Science)
Massimo Mastrangeli (TU Delft - Electrical Engineering, Mathematics and Computer Science)
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Abstract
We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.