Recording Neuronal Activity On Chip with Segmented 3D Microelectrode Arrays

Conference paper (2022)

Authors

N. Revyn Electronic Components, Technology and Materials - EEMCS
Michel Hu Leiden University Medical Center
Jean Philippe Frimat Leiden University Medical Center
B. de Wagenaar Electronic Components, Technology and Materials - EEMCS
Arn M.J.M. van den Maagdenberg Leiden University Medical Center
Pasqualina M Sarro Electronic Components, Technology and Materials - EEMCS
Massimo Mastrangeli Electronic Components, Technology and Materials - EEMCS
Research Group
Electronic Components, Technology and Materials (EEMCS) (TU Delft)
Copyright: © 2022 N. Revyn, Michel H.Y. Hu, Jean-Philippe Frimat, B. de Wagenaar, Arn M.J.M. van den Maagdenberg, Pasqualina M Sarro, Massimo Mastrangeli
DOI: https://doi.org/10.1109/MEMS51670.2022.9699597
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Published Date

2022

Language

English

Reuse Rights

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Faculty

Electrical Engineering, Mathematics and Computer Science

Department

Microelectronics

Research Group

Electronic Components, Technology and Materials

Abstract

We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 μm-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.

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