Engineering and integration of pathways for anaerobic redox-cofactor balancing in yeast
Aafke C.A. van Aalst (TU Delft - BT/Industriele Microbiologie)
Jack Pronk – Promotor (TU Delft - BT/Biotechnologie)
J.G. Daran – Promotor (TU Delft - BT/Industriele Microbiologie)
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Abstract
The production of ethanol by the yeast Saccharomyces cerevisiae remains the process in industrial biotechnology with the largest product volume (ca. 100 million litres annually in 2022). Carbon losses occur due to the formation of biomass and glycerol, which can account for at least 8% of the product. Under anaerobic conditions, formation of yeast biomass and glycerol are coupled via redox-cofactor balances, as a net generation of NADH during biomass formation needs to be compensated by NADH-dependent formation of glycerol from sugar. This thesis discusses redox-engineering strategies for maximizing ethanol yields on substrate.
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