Euler-Lagrange analysis towards representative down-scaling of a 22 m3 aerobic S. cerevisiae fermentation
Cees Haringa (TU Delft - ChemE/Transport Phenomena)
Amit T. Deshmukh (DSM)
Rob Mudde (TU Delft - ChemE/Transport Phenomena)
Henk Noorman (TU Delft - BT/Bioprocess Engineering)
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Abstract
With reaction timescales equal to or shorter than the circulation time, the ideal mixing assumption typically does not hold for large scale bioreactors. As a consequence large scale gradients in extra-cellular conditions such as the substrate concentration exist, which may significantly impact the metabolism of micro-organisms and thereby the process performance. The influence of extra-cellular variations on the organism can be tested using so-called scale-down simulators, laboratory scale setups where deliberate, controlled fluctuations are imposed in the extra-cellular environment.
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