Handling and Sensing of Single Enzyme Molecules

From Fluorescence Detection towards Nanoscale Electrical Measurements

Journal article (2016)

Authors

K.H. Mathwig Rijksuniversiteit Groningen
Qijin Chi Technical University of Denmark (DTU)
Serge G. Lemay University of Twente
L. Rassaei OLD ChemE/Organic Materials and Interfaces
Research Group
OLD ChemE/Organic Materials and Interfaces
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Published Date

2016

Language

English

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Research Group

OLD ChemE/Organic Materials and Interfaces

Abstract

Classical methods to study single enzyme molecules have provided valuable information about the distribution of conformational heterogeneities, reaction mechanisms, and transients in enzymatic reactions when individual molecules instead of an averaging ensemble are studied. Here, we highlight major advances in all-electrical single enzyme studies with a focus on recent micro- and nanofluidic tools, which offer new ways of handling and studying small numbers of molecules or even single enzyme molecules. We particularly emphasize nanofluidic devices, which enable the integration of electrochemical transduction and detection.