SiFLIM

Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Journal article (2016)

Authors

Marcel Raspe Netherlands Cancer Institute
Katarzyna M. Kedziora Netherlands Cancer Institute
Kees Jalink Universiteit van Amsterdam, Van Leeuwenhoek Centre for Advanced Microscopy, Netherlands Cancer Institute
Bram Van Den Broek Netherlands Cancer Institute, Universiteit van Amsterdam
Q. Zhao ImPhys/Quantitative Imaging
Sander De Jong Lambert Instruments B.V.
Johan Herz Lambert Instruments B.V.
Marieke Mastop Universiteit van Amsterdam
Joachim Goedhart Universiteit van Amsterdam, Van Leeuwenhoek Centre for Advanced Microscopy
Theodorus W.J. Gadella Van Leeuwenhoek Centre for Advanced Microscopy, Universiteit van Amsterdam
I.T. Young ImPhys/Quantitative Imaging
Research Group
ImPhys/Quantitative Imaging
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Published Date

2016

Language

English

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Research Group

ImPhys/Quantitative Imaging

Abstract

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.