IY
I.T. Young
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10 records found
1
SiFLIM
Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data
Journal article
(2016)
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Marcel Raspe, Katarzyna M. Kedziora, Kees Jalink, Bram Van Den Broek, Qiaole Zhao, Sander De Jong, Johan Herz, Marieke Mastop, Joachim Goedhart, Theodorus W.J. Gadella, Ian T. Young
We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
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We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.
Conference paper
(2012)
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Q Zhao, IT Young, K Jalink, S de Jong, B van Geest, KWJ Stoop, B Schelen, RN Schouten, R Oever, R Leenen, HC van Kuijk, I Peters, FJ Polderdijk, JT Bosiers