We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.@en

Reducing waiting times is crucial for ports to be efficient and competitive. Important causes of waiting times are cascading interactions between realistic hydrodynamics, accessibility policies, vessel-priority rules, and detailed berth availability. The main challenges are determining the cause of waiting and finding rational solutions to reduce waiting time. In this study, we focus on the role of the design depth of a channel on the waiting times. We quantify the performance of channel depth for a representative fleet rather than the common approach of a single normative design vessel. The study relies on a mesoscale agent-based discrete-event model that can take processed Automatic Identification System and hydrodynamic data as its main input. The presented method’s validity is assessed by hindcasting one year of observed anchorage area laytimes for a liquid bulk terminal in the Port of Rotterdam. The hindcast demonstrates that the method predicts the causes of 73.4% of the non-excessive laytimes of vessels, thereby correctly modelling 60.7% of the vessels-of-call. Following a recent deepening of the access channel, cascading waiting times due to tidal restrictions were found to be limited. Nonetheless, the importance of our approach is demonstrated by testing alternative maintained bed level designs, revealing the method’s potential to support rational decision-making in coastal zones.@en