TG

Theodorus W.J. Gadella

1 records found

SiFLIM

Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and i ...