Joachim Goedhart

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Journal article (1)

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SiFLIM

Single-image frequency-domain FLIM provides fast and photon-efficient lifetime data

Journal article (2016) - Marcel Raspe , Katarzyna M. Kedziora , Kees Jalink , Bram Van Den Broek , Qiaole Zhao , Sander De Jong , Johan Herz , Marieke Mastop , Joachim Goedhart , Theodorus W.J. Gadella , Ian T. Young

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and i ...