Designing biocatalytic redox reactions with oxidoreductases for organic chemistry

Doctoral thesis (2020)

Authors

M.C.R. Rauch BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2020 M.C.R. Rauch
DOI: https://doi.org/10.4233/uuid:afcbf229-f6ba-4b81-a561-6a0a61dc74de
More Info
expand_more

Published Date

07-02-2020

Language

English

Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

The focus of this thesis is to identify improvements of oxidoreductase reactions to bring them to preparative scale. Three approaches were studied: (1) photoregeneration of oxidised nicotinamide cofactors with LEDs as light source and flavins, (2) direct regeneration of Old Yellow Enzymes with light or metals, (3) heme-thiolate enzyme peroxygenases in neat substrate conditions.
Flavins are the main actors of the first two approaches. These redox cofactors have been chosen for their photocatalytic properties as a photosensitiser and as a cofactor in the so-called flavoproteins.