CRISPR-Cas Systems Reduced to a Minimum
Cristóbal Almendros (TU Delft - BN/Stan Brouns Lab, Kavli institute of nanoscience Delft)
Sebastian N. Kieper (Kavli institute of nanoscience Delft, TU Delft - BN/Stan Brouns Lab)
Stan J.J. Brouns (TU Delft - BN/Stan Brouns Lab, Kavli institute of nanoscience Delft, Wageningen University & Research)
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Abstract
In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.
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