Enantioselective Sulfoxidation of Thioanisole by Cascading a Choline Oxidase and a Peroxygenase in the Presence of Natural Deep Eutectic Solvents
Yongru Li (South China University of Technology)
Yunjian Ma (South China University of Technology)
Peilin Li (South China University of Technology)
Xizhen Zhang (South China University of Technology)
Doris Ribitsch (acib GmbH)
Miguel Alcalde (Institute of Catalysis, CSIC, Madrid)
Frank Hollmann (TU Delft - BT/Biocatalysis)
Yonghua Wang (South China University of Technology)
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Abstract
A bienzymatic cascade for selective sulfoxidation is presented. The evolved recombinant peroxygenase from Agrocybe aegeritra catalyses the enantioselective sulfoxidation of thioanisole whereas the choline oxidase from Arthrobacter nicotianae provides the H2 O2 necessary via reductive activation of ambient oxygen. The reactions are performed in choline chloride-based deep eutectic solvents serving as co-solvent and stoichiometric reductant at the same time. Very promising product concentrations (up to 15 mM enantiopure sulfoxide) and catalyst performances (turnover numbers of 150,000 and 2100 for the peroxygenase and oxidase, respectively) have been achieved.
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