Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration
Andreas Tosstorff (DECHEMA Research Institute)
Cora Kroner (DECHEMA Research Institute)
Diederik J. Opperman (University of the Free State)
Frank Hollmann (TU Delft - BT/Biocatalysis)
Dirk Holtmann (DECHEMA Research Institute)
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Abstract
Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.