Expansion Microscopy for Nanoscale Imaging of the Nervous System in Health and Disease
H.E.M.A. Abbady (TU Delft - Mechanical Engineering)
Z. Gao – Mentor (TU Delft - Biomechanical Engineering)
Vera van Dis – Mentor (Erasmus MC)
Johan Slotman – Graduation committee member (Erasmus MC)
H.J. Geertsema – Mentor (TU Delft - ImPhys/Geertsema group)
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Abstract
Expansion microscopy (ExM) is a new approach to super-resolution that relies on the physical isotropic expansion of a sample in a swellable polymer network. We developed a novel ExM method termed Sevenfold Post-labelling Expansion Microscopy (SP-ExM) that enables sevenfold expansion in a single step of thick cerebellar mouse brain and human tumour and peritumoral cortex samples, while preserving proteins for bulk staining of the entire proteome. Using SP-ExM, we localized proteins with nanoscale precision in their ultrastructural context without any specialized procedures or equipment. As such, SP-ExM contributes to better understanding the ultrastructural layout of specific proteins in health and disease.
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File under embargo until 24-04-2026