In this paper, we present an equivalent circuit model that integrates a living myocardial slice (LMS) cultured on a microelectrode array (MEA) to effectively simulates a heart-on-a-chip (HoC) within Electronic Design Automation (EDA) software. The cardiac fiber model consists of cardiomyocytes interconnected by gap junctions to simulate the action potential (AP) conduction in the longitudinal direction. We systematically explored several parameters, including gap junction resistors, seal resistors, and electrode diameters, to assess their effects on local field potential (LFP). The model accuracy was validated through in vitro experiments using mouse LMS, confirming its potential for guiding HoC design in cardiac research.