An Equivalent Circuit Model of Living Myocardial Slice Cultured on Microelectrode Array with in-vitro Experimental Validations
Rui Guan (Erasmus MC, TU Delft - Electrical Engineering, Mathematics and Computer Science, NXP Semiconductors)
Tao Shen (TU Delft - Electrical Engineering, Mathematics and Computer Science)
Paul Knops (Erasmus MC)
Yannick J.H.J. Taverne (Erasmus MC)
Zhenyu Gao (Erasmus MC, TU Delft - Mechanical Engineering)
Sijun Du (TU Delft - Electrical Engineering, Mathematics and Computer Science)
Robert Van Veldhoven (NXP Semiconductors, TU Delft - Electrical Engineering, Mathematics and Computer Science)
Natasja M.S. De Groot (Erasmus MC, TU Delft - Mechanical Engineering, TU Delft - Electrical Engineering, Mathematics and Computer Science)
Frans Widdershoven (NXP Semiconductors, TU Delft - Electrical Engineering, Mathematics and Computer Science)
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Abstract
In this paper, we present an equivalent circuit model that integrates a living myocardial slice (LMS) cultured on a microelectrode array (MEA) to effectively simulates a heart-on-a-chip (HoC) within Electronic Design Automation (EDA) software. The cardiac fiber model consists of cardiomyocytes interconnected by gap junctions to simulate the action potential (AP) conduction in the longitudinal direction. We systematically explored several parameters, including gap junction resistors, seal resistors, and electrode diameters, to assess their effects on local field potential (LFP). The model accuracy was validated through in vitro experiments using mouse LMS, confirming its potential for guiding HoC design in cardiac research.