Cellular balancing under dynamic conditions

A systems biology-based discovery using experimental and modelling approaches

Doctoral thesis (2023)

Authors

K.J.A. Verhagen BT/Industriele Microbiologie - AS
Research Group
BT/Industriele Microbiologie (AS) (TU Delft)
Copyright: © 2023 K.J.A. Verhagen
DOI
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https://doi.org/10.4233/uuid:d43f7180-be04-4e5b-a090-993f52433513
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Published Date

2023

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Industriele Microbiologie

Abstract

Saccharomyces cerevisiae, also known as baker’s yeast, is a robust microorganism frequently used in industrial biotechnology. The scale of its applications ranges from several millilitres for research and process development in the lab to hundreds of cubic meters for cultivation in industrial production processes. In large-scale reactors mixing limitations inherently lead to physiochemical gradients in substrate and oxygen concentrations, pH or temperature. Such inhomogeneous environment in production processes can cause a reduced yield or titer compared to the small-scale development processes. Such scale performance differences can lead to significant worse process economics and increase costs and development time.
The scope of this thesis is to study and understand the regulation of Saccharomyces cerevisiae metabolism under dynamic substrate conditions, using both experimental and modelling approaches.